Linked Life Data

8432811

Source:http://linkedlifedata.com/resource/pubmed/id/8432811

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1993-3-16
pubmed:abstractText
The confirmation of infection with human T-cell lymphotropic virus type I (HTLV-I) and type II (HTLV-II) currently involves multiple assays. These include Western blot (immunoblot) (WB) and/or radioimmunoprecipitation assay for detection of antibodies to HTLV-specific viral proteins and polymerase chain reaction and/or peptide-based enzyme immunoassays for differentiating between the two viruses. We undertook an evaluation of a modified WB assay that includes native HTLV-I viral proteins from MT-2 cells spiked with an HTLV-I transmembrane glycoprotein (recombinant p21e) and the HTLV-I- and HTLV-II-specific recombinant proteins MTA-1 and K55. The test panel consisted of well-characterized sera from U.S. blood donors, American Indians, intravenous drug users, and patients seen in sexually transmitted disease clinics. Of 158 HTLV-I/II-seropositive serum specimens tested, 156 (98.7%) were confirmed and typed as HTLV-I or HTLV-II. Of 82 HTLV-I/II-seroindeterminate or -seronegative serum specimens, only 1 was classified as HTLV-II positive: the sample had weak gag p19 and strong gag p24 and env p21e reactivity and was radioimmunoprecipitation assay negative for env gp61/68 but polymerase chain reaction positive for HTLV-II. The specificity of the modified WB for confirming and typing serum samples was therefore 100%. We conclude that this WB assay is useful for confirming and typing HTLV infection and can help simplify HTLV-I/II testing algorithms.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8432811-1347047, http://linkedlifedata.com/resource/pubmed/commentcorrection/8432811-1370526, http://linkedlifedata.com/resource/pubmed/commentcorrection/8432811-1548575, http://linkedlifedata.com/resource/pubmed/commentcorrection/8432811-1551991, http://linkedlifedata.com/resource/pubmed/commentcorrection/8432811-1729620, http://linkedlifedata.com/resource/pubmed/commentcorrection/8432811-1856487, http://linkedlifedata.com/resource/pubmed/commentcorrection/8432811-1864928, http://linkedlifedata.com/resource/pubmed/commentcorrection/8432811-1939580, http://linkedlifedata.com/resource/pubmed/commentcorrection/8432811-1972466, http://linkedlifedata.com/resource/pubmed/commentcorrection/8432811-1992314, http://linkedlifedata.com/resource/pubmed/commentcorrection/8432811-2185257, http://linkedlifedata.com/resource/pubmed/commentcorrection/8432811-2247455, http://linkedlifedata.com/resource/pubmed/commentcorrection/8432811-2378978, http://linkedlifedata.com/resource/pubmed/commentcorrection/8432811-2790192
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
31
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
260-4
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Evaluation of an immunoblot assay for serological confirmation and differentiation of human T-cell lymphotropic virus types I and II.
pubmed:affiliation
Division of Viral and Rickettsial Diseases, Centers for Disease Control, Atlanta, Georgia 30333.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.