Linked Life Data

8431426

Source:http://linkedlifedata.com/resource/pubmed/id/8431426

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1993-3-15
pubmed:abstractText
The replacement of Lys258 by alanine (K258A) in aspartate aminotransferase reduces the rate constant for the central, 1,3-prototropic shift by 10(6)-10(8)-fold, confirming the role of Lys258 as the general-base catalyst for this step. The rate constant for the 1,3-prototropic shift interconverting K258A aldimine and ketimine intermediates is pH-independent like that of the wild-type enzyme (WT-AATase). K258A binds amino acid substrates in external aldimine intermediates 10(5)-fold more tightly than does WT-AATase. The excess amino acid binding energy observed in the mutant is sacrificed by the WT-AATase in order to increase the value of kcat. The net result is that the kcat/KM values for amino acid substrates are reduced only 3-100-fold by the mutation. This provides a clear example of the Circe effect propounded by Jencks [Jencks, W. P. (1975) Adv. Enzymol. Rel. Areas Mol. Biol. 43, 219]. Part of the increase in kcat due to the inclusion of Lys258 is accomplished by a 10(4)-10(5)-fold acceleration of external aldimine formation and hydrolysis. This step is partially rate-determining for K258A, but not for WT-AATase. A significant consequence of the utilization of amino acid binding energy for catalysis is the raising of the dissociation constants for these substrates to levels near the physiological concentrations of amino acids. The major product of the reaction of K258A with oxalacetate is pyruvate due to decarboxylation of the beta-imine formed in the ketimine intermediate.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0006-2960
pubmed:author
pubmed:issnType
Print
pubmed:day
16
pubmed:volume
32
pubmed:geneSymbol
aspC
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1471-9
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Lysine 258 in aspartate aminotransferase: enforcer of the Circe effect for amino acid substrates and general-base catalyst for the 1,3-prototropic shift.
pubmed:affiliation
Department of Molecular and Cell Biology, University of California, Berkeley 94720.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.