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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1993-3-15
|
| pubmed:abstractText |
A highly efficient method of introducing DNA into an important human pathogen is reported here. Electroporation-mediated transformation of a laboratory-passaged isolate of Helicobacter pylori was successfully used to establish genetic mutants at a transformation frequency of > 10(5)/micrograms DNA. This method should be widely applicable to all isolates of H. pylori and may eliminate the variability reported when natural transformation was used on fresh clinical isolates.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0736-6205
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
14
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
225-6
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:8431287-DNA, Bacterial,
pubmed-meshheading:8431287-Drug Resistance, Microbial,
pubmed-meshheading:8431287-Electricity,
pubmed-meshheading:8431287-Helicobacter pylori,
pubmed-meshheading:8431287-Mutation,
pubmed-meshheading:8431287-Streptomycin,
pubmed-meshheading:8431287-Transformation, Bacterial
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Transformation of Helicobacter pylori by electroporation.
|
| pubmed:affiliation |
Department of Microbiology and Immunology, Stanford University, CA 94305.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|