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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1993-3-15
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pubmed:abstractText |
A hybrid transient expression system, in which tissue culture cells are infected with a recombinant vaccinia virus encoding bacteriophage DNA-dependent RNA polymerase and transfected with a plasmid containing a cloned gene behind the bacteriophage promoter, allows rapid high-level expression in nearly 100% of the cells. In order to extend this system to clones from libraries containing SP6 promoters, a new vaccinia virus was constructed encoding bacteriophage SP6 RNA polymerase.
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pubmed:language |
eng
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|
pubmed:journal |
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|
pubmed:citationSubset |
IM
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|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
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|
pubmed:month |
Feb
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|
pubmed:issn |
0736-6205
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pubmed:author |
|
|
pubmed:issnType |
Print
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pubmed:volume |
14
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pubmed:owner |
NLM
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|
pubmed:authorsComplete |
Y
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pubmed:pagination |
222-4
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:8431286-Bacteriophages,
pubmed-meshheading:8431286-Cloning, Molecular,
pubmed-meshheading:8431286-DNA, Viral,
pubmed-meshheading:8431286-DNA-Directed RNA Polymerases,
pubmed-meshheading:8431286-Gene Expression,
pubmed-meshheading:8431286-Plasmids,
pubmed-meshheading:8431286-Promoter Regions, Genetic,
pubmed-meshheading:8431286-Recombinant Proteins,
pubmed-meshheading:8431286-Transfection,
pubmed-meshheading:8431286-Vaccinia virus,
pubmed-meshheading:8431286-beta-Galactosidase
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|
pubmed:year |
1993
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|
pubmed:articleTitle |
SP6 RNA polymerase containing vaccinia virus for rapid expression of cloned genes in tissue culture.
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pubmed:affiliation |
Natl. Institute of Mental Health, National Institutes of Health, Bethesda, MD 20892.
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pubmed:publicationType |
Journal Article
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