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rdf:type |
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lifeskim:mentions |
umls-concept:C0007732,
umls-concept:C0009015,
umls-concept:C0017262,
umls-concept:C0185117,
umls-concept:C0599930,
umls-concept:C0679058,
umls-concept:C0684192,
umls-concept:C0995740,
umls-concept:C0995754,
umls-concept:C1547699,
umls-concept:C2700640,
umls-concept:C2911684
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pubmed:issue |
1
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pubmed:dateCreated |
1993-3-9
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pubmed:abstractText |
A 26-mer DNA probe was designed from N-terminal sequence data for the cephalosporin 7 alpha-hydroxylase (CH) of Streptomyces clavuligerus NRRL 3585 and used to screen a DNA library from this organism. The library was constructed in the lambda GEM-11 phage system. After plaque purification and reprobing, positive recombinant phages were chosen for further analysis. Characterization of the cloned DNA by restriction mapping and Southern hybridization showed that a 1.5-kb SalI fragment hybridized to the probe. Polymerase chain reaction assays using this fragment as a template and the probe as a primer indicated that the fragment carries the entire putative CH gene (cmcI). This was confirmed through the expression of CH enzymatic activity when the fragment was introduced into Streptomyces lividans. A putative beta-lactamase activity was detected in S. lividans.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8431021-1195397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8431021-1366440,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8431021-1706706,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8431021-1747122,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8431021-2076552,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8431021-2203307,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8431021-4670501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8431021-4780815,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8431021-4790591,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8431021-7378068,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8431021-879738
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0066-4804
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
37
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pubmed:geneSymbol |
cmcI
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
84-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8431021-Amino Acid Sequence,
pubmed-meshheading:8431021-Bacteriophages,
pubmed-meshheading:8431021-Blotting, Southern,
pubmed-meshheading:8431021-Chromatography, High Pressure Liquid,
pubmed-meshheading:8431021-Cloning, Molecular,
pubmed-meshheading:8431021-DNA, Bacterial,
pubmed-meshheading:8431021-DNA Probes,
pubmed-meshheading:8431021-Gene Expression,
pubmed-meshheading:8431021-Mixed Function Oxygenases,
pubmed-meshheading:8431021-Molecular Sequence Data,
pubmed-meshheading:8431021-Nucleic Acid Hybridization,
pubmed-meshheading:8431021-Oligonucleotides,
pubmed-meshheading:8431021-Polymerase Chain Reaction,
pubmed-meshheading:8431021-Recombination, Genetic,
pubmed-meshheading:8431021-Streptomyces
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pubmed:year |
1993
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pubmed:articleTitle |
Cloning of a Streptomyces clavuligerus DNA fragment encoding the cephalosporin 7 alpha-hydroxylase and its expression in Streptomyces lividans.
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pubmed:affiliation |
Department of Biology, Northeastern University, Boston, Massachusetts 02115.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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