| pubmed-article:8428965 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8428965 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:8428965 | lifeskim:mentions | umls-concept:C0231337 | lld:lifeskim |
| pubmed-article:8428965 | lifeskim:mentions | umls-concept:C0016030 | lld:lifeskim |
| pubmed-article:8428965 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:8428965 | lifeskim:mentions | umls-concept:C0012568 | lld:lifeskim |
| pubmed-article:8428965 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:8428965 | lifeskim:mentions | umls-concept:C1336633 | lld:lifeskim |
| pubmed-article:8428965 | lifeskim:mentions | umls-concept:C0205369 | lld:lifeskim |
| pubmed-article:8428965 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:8428965 | lifeskim:mentions | umls-concept:C1314939 | lld:lifeskim |
| pubmed-article:8428965 | lifeskim:mentions | umls-concept:C0301039 | lld:lifeskim |
| pubmed-article:8428965 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:8428965 | pubmed:dateCreated | 1993-3-8 | lld:pubmed |
| pubmed-article:8428965 | pubmed:abstractText | The aging of IMR-90 human diploid fibroblasts in culture is accompanied by a 5-7 fold decrease in the level of thymidine kinase (TK) mRNA and TK activity (Chang, Z. F., and Chen, K. Y. (1988) J. Biol. Chem. 263, 11431-11435). We have employed a gel mobility shift analysis to investigate the molecular basis of the age-dependent attenuation of TK gene expression. Several cis-elements including two inverted CCAAT boxes, located at base pairs (bp) -36 and -67, and GC-rich Sp1 binding sites have been identified in the TK promoter. A 28-bp (-91 to -64) fragment containing the distal inverted CCAAT element was excised from the TK promoter to examine possible differences in nuclear protein binding between young and old IMR-90 cells. A prominent DNA-protein complex was identified in serum-stimulated young cells by a gel mobility shift assay. Competition analysis indicated that the binding was highly specific. The nuclear protein responsible for the complex formation was named CBP/tk (CCAAT Binding Protein for TK gene) since methylation interference assay showed that the inverted CCAAT box was involved in binding. The appearance of the CBP/tk-28-bp complex in IMR-90 cells was (i) serum-dependent, becoming prominent 12-24 h after serum stimulation, and (ii) age-dependent, prominent only in young but not in old IMR-90 cells. Similar serum- and age-dependent complex formations were also observed using a 67-bp fragment (-63 to +4) containing the proximal CCAAT element and a TATA box. In contrast, the binding activities for the Sp1 sequence were the same in young and old cells and appeared to be serum-independent. CBP/tk binding activity in nuclear extracts was abolished by heat (60 degrees C, 5 min) or treatment with proteinase K (0.1 microgram/ml) and sodium dodecyl sulfate (0.005%), but not by Nonidet P-40 or Triton X-100. Treatment of nuclear extracts with alkaline phosphatase or lectins (concanavalin A and wheat germ agglutinin) did not affect the binding activity. Metal chelators such as 1,10-ortho-phenanthroline (0.5 mM) inhibited the CBP/tk binding activity. Cycloheximide added to the serum-stimulated cultures at an early or mid-G1 phase inhibited the CBP/tk binding activity. The half-life of the serum-induced CBP/tk binding activity was estimated to be less than 1 h.(ABSTRACT TRUNCATED AT 400 WORDS) | lld:pubmed |
| pubmed-article:8428965 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8428965 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8428965 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8428965 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8428965 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8428965 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:8428965 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8428965 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:8428965 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8428965 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:8428965 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:8428965 | pubmed:author | pubmed-author:ChenK YKY | lld:pubmed |
| pubmed-article:8428965 | pubmed:author | pubmed-author:PangJ HJH | lld:pubmed |
| pubmed-article:8428965 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8428965 | pubmed:day | 5 | lld:pubmed |
| pubmed-article:8428965 | pubmed:volume | 268 | lld:pubmed |
| pubmed-article:8428965 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8428965 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8428965 | pubmed:pagination | 2909-16 | lld:pubmed |
| pubmed-article:8428965 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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| pubmed-article:8428965 | pubmed:meshHeading | pubmed-meshheading:8428965-... | lld:pubmed |
| pubmed-article:8428965 | pubmed:year | 1993 | lld:pubmed |
| pubmed-article:8428965 | pubmed:articleTitle | A specific CCAAT-binding protein, CBP/tk, may be involved in the regulation of thymidine kinase gene expression in human IMR-90 diploid fibroblasts during senescence. | lld:pubmed |
| pubmed-article:8428965 | pubmed:affiliation | Department of Chemistry, Rutgers University, Piscataway, New Jersey 08855-0939. | lld:pubmed |
| pubmed-article:8428965 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8428965 | pubmed:publicationType | In Vitro | lld:pubmed |
| pubmed-article:8428965 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:8428965 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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