Linked Life Data

8428965

Source:http://linkedlifedata.com/resource/pubmed/id/8428965

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1993-3-8
pubmed:abstractText
The aging of IMR-90 human diploid fibroblasts in culture is accompanied by a 5-7 fold decrease in the level of thymidine kinase (TK) mRNA and TK activity (Chang, Z. F., and Chen, K. Y. (1988) J. Biol. Chem. 263, 11431-11435). We have employed a gel mobility shift analysis to investigate the molecular basis of the age-dependent attenuation of TK gene expression. Several cis-elements including two inverted CCAAT boxes, located at base pairs (bp) -36 and -67, and GC-rich Sp1 binding sites have been identified in the TK promoter. A 28-bp (-91 to -64) fragment containing the distal inverted CCAAT element was excised from the TK promoter to examine possible differences in nuclear protein binding between young and old IMR-90 cells. A prominent DNA-protein complex was identified in serum-stimulated young cells by a gel mobility shift assay. Competition analysis indicated that the binding was highly specific. The nuclear protein responsible for the complex formation was named CBP/tk (CCAAT Binding Protein for TK gene) since methylation interference assay showed that the inverted CCAAT box was involved in binding. The appearance of the CBP/tk-28-bp complex in IMR-90 cells was (i) serum-dependent, becoming prominent 12-24 h after serum stimulation, and (ii) age-dependent, prominent only in young but not in old IMR-90 cells. Similar serum- and age-dependent complex formations were also observed using a 67-bp fragment (-63 to +4) containing the proximal CCAAT element and a TATA box. In contrast, the binding activities for the Sp1 sequence were the same in young and old cells and appeared to be serum-independent. CBP/tk binding activity in nuclear extracts was abolished by heat (60 degrees C, 5 min) or treatment with proteinase K (0.1 microgram/ml) and sodium dodecyl sulfate (0.005%), but not by Nonidet P-40 or Triton X-100. Treatment of nuclear extracts with alkaline phosphatase or lectins (concanavalin A and wheat germ agglutinin) did not affect the binding activity. Metal chelators such as 1,10-ortho-phenanthroline (0.5 mM) inhibited the CBP/tk binding activity. Cycloheximide added to the serum-stimulated cultures at an early or mid-G1 phase inhibited the CBP/tk binding activity. The half-life of the serum-induced CBP/tk binding activity was estimated to be less than 1 h.(ABSTRACT TRUNCATED AT 400 WORDS)
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
5
pubmed:volume
268
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2909-16
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:8428965-Base Sequence, pubmed-meshheading:8428965-Binding, Competitive, pubmed-meshheading:8428965-CCAAT-Enhancer-Binding Proteins, pubmed-meshheading:8428965-Cell Aging, pubmed-meshheading:8428965-Cell Cycle, pubmed-meshheading:8428965-Cells, Cultured, pubmed-meshheading:8428965-Chelating Agents, pubmed-meshheading:8428965-Cycloheximide, pubmed-meshheading:8428965-DNA-Binding Proteins, pubmed-meshheading:8428965-Detergents, pubmed-meshheading:8428965-Gene Expression Regulation, Enzymologic, pubmed-meshheading:8428965-Humans, pubmed-meshheading:8428965-Methylation, pubmed-meshheading:8428965-Molecular Sequence Data, pubmed-meshheading:8428965-Nuclear Proteins, pubmed-meshheading:8428965-Oligodeoxyribonucleotides, pubmed-meshheading:8428965-Thymidine Kinase, pubmed-meshheading:8428965-Transcription Factors
pubmed:year
1993
pubmed:articleTitle
A specific CCAAT-binding protein, CBP/tk, may be involved in the regulation of thymidine kinase gene expression in human IMR-90 diploid fibroblasts during senescence.
pubmed:affiliation
Department of Chemistry, Rutgers University, Piscataway, New Jersey 08855-0939.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't