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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1993-3-10
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pubmed:abstractText |
The secretion of the Klebsiella oxytoca cell surface lipoprotein pullulanase involves translocation across the cytoplasmic and outer membranes of the Gram-negative bacterial cell envelope. A variant of pullulanase was created by fusing the signal peptide-encoding 5' region of the Escherichia coli gene for periplasmic MalE protein to the 3' end of the pulA gene encoding almost the entire mature part of pullulanase. When produced in E. coli carrying the malE-pulA gene fusion on a high copy number plasmid and the complete set of genes specifically required for pullulanase secretion on a second plasmid, the hybrid protein differed from wild-type pullulanase as follows: (i) it was not fatty-acylated; (ii) it was apparently processed by LepB signal peptidase rather than by LspA lipoprotein signal peptidase; (iii) it was released into the periplasm and was only slowly transported across the outer membrane, and (iv) it was released directly into the medium rather than via the usual surface-anchored intermediate. The hybrid protein was secreted more rapidly when malE-pulA was expressed from a low copy number plasmid. The two steps in the secretion pathway could be totally uncoupled by expressing first the malE-pulA gene fusion and then the cognate secretion genes. These results show that fatty-acylation of wild-type PulA is not essential for secretion but may improve its efficiency when large amounts of the protein are produced, that the two steps in secretion can occur quite independently and that the periplasmic intermediate can persist for long periods under certain circumstances.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8428585-101676,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8428585-1313415,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8428585-1373213,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8428585-1574004,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8428585-1588814,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8428585-1640836,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8428585-1683764,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8428585-1738317,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8428585-1740115,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8428585-1829728,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8428585-1856199,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8428585-1856217,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8428585-1857208,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8428585-1934062,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8428585-1944223,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8428585-1992458,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8428585-2041472,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8428585-2088168,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8428585-2088181,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8428585-2124971,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8428585-2181241,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8428585-2181242,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8428585-2233249,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8428585-2406254,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8428585-2495264,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8428585-2538630,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8428585-2661532,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8428585-2668690,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8428585-2677008,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8428585-2693596,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8428585-2845225,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8428585-3011607,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8428585-3322811,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8428585-3327753,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8428585-3519575,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8428585-3902791,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8428585-3972770,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8428585-4298222,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8428585-6389513
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/ATP-Binding Cassette Transporters,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Escherichia coli Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Glycoside Hydrolases,
http://linkedlifedata.com/resource/pubmed/chemical/MalE protein, E coli,
http://linkedlifedata.com/resource/pubmed/chemical/Maltose-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Monosaccharide Transport Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Oligodeoxyribonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Periplasmic Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Sorting Signals,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/maltose transport system, E coli,
http://linkedlifedata.com/resource/pubmed/chemical/pullulanase
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0261-4189
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
12
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pubmed:geneSymbol |
malE,
pulA
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
271-8
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:8428585-Molecular Weight,
pubmed-meshheading:8428585-Kinetics,
pubmed-meshheading:8428585-Klebsiella,
pubmed-meshheading:8428585-Escherichia coli,
pubmed-meshheading:8428585-Glycoside Hydrolases,
pubmed-meshheading:8428585-Membrane Proteins,
pubmed-meshheading:8428585-Cell Membrane,
pubmed-meshheading:8428585-Base Sequence,
pubmed-meshheading:8428585-Time Factors,
pubmed-meshheading:8428585-Genes, Bacterial,
pubmed-meshheading:8428585-Amino Acid Sequence,
pubmed-meshheading:8428585-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:8428585-Molecular Sequence Data,
pubmed-meshheading:8428585-Monosaccharide Transport Proteins,
pubmed-meshheading:8428585-Carrier Proteins,
pubmed-meshheading:8428585-Escherichia coli Proteins,
pubmed-meshheading:8428585-Cloning, Molecular,
pubmed-meshheading:8428585-Oligodeoxyribonucleotides
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