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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1993-3-9
|
| pubmed:abstractText |
Sera from 75 patients with clinical type I allergy against dogs were investigated by means of immunoblotting using extracts prepared from dog hair/dander (CAN XI D) and saliva. In addition, selected sera were tested on extracts made of hair, skin, salivary glands (parotis and submandibularis), serum and liver. A 69-kD IgE-binding protein was identified in all extracts tested with an incidence of approximately 40% and shown to be dog albumin by means of inhibition experiments. In 96% of patients' sera IgE antibodies reactive with a 19-kD and/or a 23-kD protein of the hair/dander extract (CAN XI D) were observed. IgE binding to a 23-kD band was also detected in the hair and saliva extracts, but not in skin, salivary gland, serum and liver extracts. A 19-kD IgE-binding protein was strongly expressed in skin and to a lesser degree in saliva, but not in hair, serum and liver. Preincubation of patients sera with the hair extract and subsequent probing with the hair/dander extract (CAN XI D) inhibited IgE binding to the 23 kD protein whereas preincubation with the skin extract abolished IgE binding to the 19-kD protein. Using the hair/dander extract as inhibitor, IgE binding to the 19- and 23-kD proteins of saliva was abrogated. Thus it is concluded that the 23-kD protein is preferentially expressed in hair and saliva whereas the 19-kD protein is found in saliva and skin. Furthermore these two proteins are likely to represent immunologically independent major allergens.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
1018-2438
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
100
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
60-7
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8428166-Adolescent,
pubmed-meshheading:8428166-Adult,
pubmed-meshheading:8428166-Allergens,
pubmed-meshheading:8428166-Animals,
pubmed-meshheading:8428166-Antigen-Antibody Reactions,
pubmed-meshheading:8428166-Child,
pubmed-meshheading:8428166-Dogs,
pubmed-meshheading:8428166-Female,
pubmed-meshheading:8428166-Hair,
pubmed-meshheading:8428166-Humans,
pubmed-meshheading:8428166-Immunoblotting,
pubmed-meshheading:8428166-Immunoglobulin E,
pubmed-meshheading:8428166-Liver,
pubmed-meshheading:8428166-Male,
pubmed-meshheading:8428166-Middle Aged,
pubmed-meshheading:8428166-Radioallergosorbent Test,
pubmed-meshheading:8428166-Saliva,
pubmed-meshheading:8428166-Skin,
pubmed-meshheading:8428166-Tissue Extracts
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Characterisation of dog allergens by means of immunoblotting.
|
| pubmed:affiliation |
Institute of Clinical Chemistry, University of Vienna, Austria.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|