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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1993-2-24
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pubmed:abstractText |
We analyzed epsilon-globin transcription in erythroid cells and in erythroid extracts to determine the requirements for enhancer-dependent expression of this gene. Mutations that abolished GATA-1 binding at a single position in the promoter prevented interaction with enhancers, whereas elimination of a second more distal promoter GATA-1 site had no effect. Deletion or mutation of the GATA-1 sites in either the human beta-globin locus control region DNase-hypersensitive site II enhancer or the chicken beta A/epsilon-globin enhancer did not diminish the ability of the enhancers to interact with the promoter. In contrast, mutation of the AP-1/NF-E2 sites in these enhancers resulted in elimination of enhancement. In vitro transcription of these constructs was promoter dependent and was not sensitive to abolition of GATA-1 binding in the promoter, consistent with the role of GATA-1 solely as a mediator of the enhancer effect. Thus, GATA-1 regulates the response of the epsilon-globin gene to enhancers through a specific site in the promoter and requires enhancer AP-1/NF-E2 binding to transduce the enhancer effect on transcription.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423810-1373805,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423810-1408744,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423810-1549112,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423810-1559609,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423810-1569944,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423810-1570310,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423810-1577269,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423810-1668190,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423810-1731219,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423810-1850324,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423810-1924329,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423810-1946439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423810-1987478,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423810-1990287,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423810-2000371,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423810-2014222,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423810-2017165,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423810-2026603,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423810-2044960,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423810-2116990,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423810-2123346,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423810-2180450,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423810-2180585,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423810-2216720,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423810-2225071,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423810-2235483,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423810-2251502,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423810-2275826,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423810-2326182,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423810-2357965,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423810-2467208,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423810-2594805,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423810-2620825,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423810-2704733,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423810-2771941,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423810-2776214,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423810-2911469,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423810-3166139,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423810-3413070,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423810-3456593,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423810-3474625,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423810-3690667,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423810-3859838,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423810-3879975,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423810-6828386
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0270-7306
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
13
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
911-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8423810-Base Sequence,
pubmed-meshheading:8423810-Cell Line,
pubmed-meshheading:8423810-DNA,
pubmed-meshheading:8423810-DNA-Binding Proteins,
pubmed-meshheading:8423810-Enhancer Elements, Genetic,
pubmed-meshheading:8423810-Erythroid-Specific DNA-Binding Factors,
pubmed-meshheading:8423810-GATA1 Transcription Factor,
pubmed-meshheading:8423810-Globins,
pubmed-meshheading:8423810-Humans,
pubmed-meshheading:8423810-Molecular Sequence Data,
pubmed-meshheading:8423810-Mutagenesis,
pubmed-meshheading:8423810-Promoter Regions, Genetic,
pubmed-meshheading:8423810-Protein Binding,
pubmed-meshheading:8423810-Proto-Oncogene Proteins c-jun,
pubmed-meshheading:8423810-Transcription, Genetic,
pubmed-meshheading:8423810-Transcription Factors
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pubmed:year |
1993
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pubmed:articleTitle |
Enhancer-dependent transcription of the epsilon-globin promoter requires promoter-bound GATA-1 and enhancer-bound AP-1/NF-E2.
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pubmed:affiliation |
Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892.
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pubmed:publicationType |
Journal Article
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