Linked Life Data

8423363

Source:http://linkedlifedata.com/resource/pubmed/id/8423363

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1993-2-22
pubmed:abstractText
The insulin time-resolved fluoroimmunoassay (TRFIA) described here is a 'sandwich type' immunoassay using two monoclonal antibodies recognizing different epitopes of insulin. One of the antibodies is fixed to a solid phase and the other is biotinylated. After sandwich formation the biotinylated antibody is detected by time-resolved fluorometry with the fluorophore europium which is coupled to streptavidin. Serum samples of 100 microliters can be analyzed within a concentration range of 4 microIU/ml up to 500 microIU/ml. The detection limit of this assay, 3.9 microIU/ml, is comparable to the conventional radioimmunoassay and the results overall correlate well with those of the radioimmunoassay (r = 0.99). There is no detectable crossreaction with human proinsulin even using a proinsulin concentration of 2000 microIU/ml. The assay is performed with the DELFIA system (Pharmacia).
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0022-1759
pubmed:author
pubmed:issnType
Print
pubmed:day
4
pubmed:volume
157
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
197-201
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
A time-resolved fluoroimmunoassay for human insulin based on two monoclonal antibodies.
pubmed:affiliation
Medical University Clinic, Freiburg, Germany.
pubmed:publicationType
Journal Article