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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1993-2-22
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pubmed:abstractText |
Previous results with the Babesia divergens gerbil vaccination model were extended in studies with cattle. Two calves were vaccinated with culture-derived B. divergens exoantigens, and two others were treated with control supernatant; both preparations were adjuvanted with Quil-A saponin. A parasite-specific humoral response was observed after the first vaccine injection and was boosted by two succeeding vaccine injections. Sera from the two vaccinated calves immunoprecipitated eight major parasitic proteins (with molecular masses ranging between 17 and 110 kDa) whose patterns were close to those observed in gerbil vaccine assays. The cellular immune response, monitored by lymphoproliferation assays, was slightly delayed in comparison with the humoral response; a significant proliferation occurred only after the second vaccine injection. Mononuclear cell proliferation was dose dependent in the presence of (i) lysates of B. divergens-parasitized erythrocytes, (ii) exoantigens of the whole supernatant, or (iii) protective exoantigens of two low-molecular-mass fractions obtained after supernatant gel filtration chromatography. An infectious challenge was administered 3 weeks after the third vaccine injection, with 3.6 x 10(10) B. divergens-parasitized erythrocytes. Erythrocyte count, rectal temperature, and parasitemia of the animals were monitored daily until they returned to initial values. All parameters indicated that the exoantigens induced protection from B. divergens infection for the two vaccinated calves.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423099-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423099-1713201,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423099-1756315,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423099-1819366,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423099-1944417,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423099-1944418,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423099-1994368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423099-2050406,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423099-2254031,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423099-230765,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423099-2435796,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423099-2482443,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423099-2528508,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423099-3325981,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423099-3410541,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423099-3439004,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423099-3944282,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423099-3997349,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423099-4090242,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423099-4433074,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/8423099-6730310,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423099-7098242,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423099-7209532,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423099-7212429,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8423099-763361
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0019-9567
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
61
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
734-41
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8423099-Animals,
pubmed-meshheading:8423099-Antibodies, Protozoan,
pubmed-meshheading:8423099-Antigens, Protozoan,
pubmed-meshheading:8423099-Babesia,
pubmed-meshheading:8423099-Babesiosis,
pubmed-meshheading:8423099-Cattle,
pubmed-meshheading:8423099-Female,
pubmed-meshheading:8423099-Hematocrit,
pubmed-meshheading:8423099-Humans,
pubmed-meshheading:8423099-Lymphocyte Activation,
pubmed-meshheading:8423099-Protozoan Vaccines,
pubmed-meshheading:8423099-Vaccination
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pubmed:year |
1993
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pubmed:articleTitle |
Cellular and humoral immune responses induced in cattle by vaccination with Babesia divergens culture-derived exoantigens correlate with protection.
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pubmed:affiliation |
Laboratoire de Biologie Cellulaire, URA Centre National de la Recherche Scientifique 290, Poitiers, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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