Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1993-2-25
pubmed:abstractText
The pilE gene from Neisseria gonorrhoeae, unlike other type-4 pilin-encoding genes, is well expressed in Escherichia coli. Two putative promoters have been implicated in the transcription of this gene. Besides the -24/-12 promoter used to transcribe type-4 pilin-encoding genes in most species, the consensus sequence for a conventional promoter is also present. The two promoters overlap and would have almost identical transcription start points (tsp). Transcription from a -24/-12 promoter should be abolished in an E. coli rpoN mutant. A recombinant plasmid carrying pilE could not be transformed into such a mutant, apparently because the synthesis of the N-terminal hydrophobic domain of pilin is lethal to the rpoN mutant. This suggests that pilE is expressed at a higher level in an rpoN mutant than it is in a wild-type (wt) strain of E. coli. This suggestion was confirmed by constructing fusions between the pilE promoter region and a promoter-less cat gene. We suggest that the conventional promoter is primarily responsible for the transcription of pilE, but that the binding of the RpoN sigma factor partially represses transcription of this gene in wt strains. In an rpoN mutant, the repression is removed and transcription occurs at a level that is lethal to the mutant host.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0378-1119
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
123
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
45-50
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:8423003-Bacterial Outer Membrane Proteins, pubmed-meshheading:8423003-Base Sequence, pubmed-meshheading:8423003-Cloning, Molecular, pubmed-meshheading:8423003-DNA, Bacterial, pubmed-meshheading:8423003-DNA-Binding Proteins, pubmed-meshheading:8423003-DNA-Directed RNA Polymerases, pubmed-meshheading:8423003-Escherichia coli, pubmed-meshheading:8423003-Escherichia coli Proteins, pubmed-meshheading:8423003-Fimbriae Proteins, pubmed-meshheading:8423003-Gene Expression Regulation, Bacterial, pubmed-meshheading:8423003-Molecular Sequence Data, pubmed-meshheading:8423003-Mutation, pubmed-meshheading:8423003-Neisseria gonorrhoeae, pubmed-meshheading:8423003-Promoter Regions, Genetic, pubmed-meshheading:8423003-RNA Polymerase Sigma 54, pubmed-meshheading:8423003-Repressor Proteins, pubmed-meshheading:8423003-Sigma Factor, pubmed-meshheading:8423003-Transformation, Bacterial
pubmed:year
1993
pubmed:articleTitle
Control of gonococcal pilin-encoding gene expression in Escherichia coli.
pubmed:affiliation
Department of Microbiology, Monash University, Clayton, Victoria, Australia.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't