Linked Life Data

8422653

Source:http://linkedlifedata.com/resource/pubmed/id/8422653

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1993-2-22
pubmed:abstractText
We have investigated a series of ovarian tumours for evidence of mutations in the p53 tumour suppressor gene. In this study we have made use of the chemical mismatch cleavage technique which, from analyses of other genes, has been shown to consistently identify all point mutations present within a region of DNA. This approach revealed mutations of p53 in 11/20 tumours studied, mainly in exons 5 or 7. After sequencing the relevant regions of the gene it was shown that ten of these mutations would have resulted in an amino acid substitution in the protein and only one represented a polymorphism. The observed incidence of p53 missense mutations in our series (50%) was the highest recorded in ovarian tumours and demonstrated the potential of the mismatch cleavage technique as a reliable method for the detection of p53 mutations in human tumours.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0304-3835
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
68
pubmed:geneSymbol
p53
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
83-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
High incidence of mutations of the p53 gene detected in ovarian tumours by the use of chemical mismatch cleavage.
pubmed:affiliation
Department of Clinical Oncology, Royal Hallamshire Hospital, Sheffield, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't