Linked Life Data

8420994

Source:http://linkedlifedata.com/resource/pubmed/id/8420994

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1993-2-18
pubmed:databankReference
pubmed:abstractText
The c-myb protooncogene is preferentially expressed in hematopoietic cells and is required for cell cycle progression at the G1/S boundary. Because c-myb encodes a transcriptional activator that functions via DNA binding, it is likely that c-myb exerts its biological activity by regulating the transcription of genes required for DNA synthesis and cell cycle progression. One such gene, cdc2, encodes a 34-kDa serine-threonine kinase that appears to be required for G1/S transition in normal human T-lymphocytes. To determine whether c-myb is a transcriptional regulator of cdc2 expression, we subcloned a segment of a cdc2 human genomic clone containing extensive 5'-flanking sequences and part of the first exon. Sequence analysis revealed the presence of two closely spaced Myb binding sites that interact with bacterially synthesized Myb protein within a region extending from nucleotides -410 to -392 upstream of the transcription initiation site. A 465-base pair segment of 5'-flanking sequence containing these sites was linked to the CAT gene and had promoter activity in rodent fibroblasts. Cotransfection of this construct with a full-length human c-myb cDNA driven by the early simian virus 40 promoter resulted in a 6-8-fold enhancement of CAT activity that was abrogated by mutations in the Myb binding sites. These data suggest that c-myb participates in the regulation of cell cycle progression by activating the expression of the cdc2 gene.
pubmed:grant
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
268
pubmed:geneSymbol
c-myb, cdc2
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2255-9
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:8420994-Animals, pubmed-meshheading:8420994-Base Sequence, pubmed-meshheading:8420994-Binding Sites, pubmed-meshheading:8420994-CDC2 Protein Kinase, pubmed-meshheading:8420994-Cell Line, pubmed-meshheading:8420994-Chloramphenicol O-Acetyltransferase, pubmed-meshheading:8420994-DNA, pubmed-meshheading:8420994-Gene Expression, pubmed-meshheading:8420994-Humans, pubmed-meshheading:8420994-Mice, pubmed-meshheading:8420994-Molecular Sequence Data, pubmed-meshheading:8420994-Mutagenesis, pubmed-meshheading:8420994-Polymerase Chain Reaction, pubmed-meshheading:8420994-Proto-Oncogene Proteins, pubmed-meshheading:8420994-Proto-Oncogene Proteins c-myb, pubmed-meshheading:8420994-RNA, Messenger, pubmed-meshheading:8420994-Recombinant Fusion Proteins, pubmed-meshheading:8420994-Transcriptional Activation, pubmed-meshheading:8420994-Transfection
pubmed:year
1993
pubmed:articleTitle
c-myb transactivates cdc2 expression via Myb binding sites in the 5'-flanking region of the human cdc2 gene.
pubmed:affiliation
Department of Microbiology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't