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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1993-2-5
|
| pubmed:abstractText |
The effects of pulsatile and steady fluid flow on the mRNA levels of proto-oncogenes c-fos, c-jun, and c-myc in cultured human umbilical vein endothelial cells (HUVEC) were investigated. c-fos mRNA levels in stationary cultures were very low. A 1 Hz pulsatile flow with an average shear stress of 16 dynes/cm2 induced a dramatic increase of c-fos mRNA levels in HUVEC 0.5 h after the onset of flow, which declined rapidly to basal levels within 1 h. Steady flow with a similar shear stress also induced a transient increase of c-fos mRNA levels, but to a lesser extent. In addition, increased c-fos mRNA levels were observed when low shear (2-6 dynes/cm2) was replaced by high shear (16-33 dynes/cm2). Pulsatile and steady flow caused a slight increase of c-jun and c-myc mRNA levels. The role of pulsatility was also investigated in platelet-derived growth factor (PDGF) expression. Pulsatile flow induced a transient increase of PDGF A- and B-chain mRNA levels with peaks at 1.5-2 h. Pulsatile flow, which was more stimulatory in mediating c-fos expression, however, was less stimulatory than steady flow in mediating PDGF expression. By using various inhibitors, protein kinase C was found to be an important mediator in flow-induced c-fos expression, with the involvement of G proteins, phospholipase C, and intracellular calcium. Protein kinase C was previously shown as a possible major mediator in flow-induced PDGF expression which, at least partly, appeared to follow the induction mechanism of c-fos, suggesting a possible connection between c-fos and PDGF induction. However, the c-fos antisense treatment, which significantly inhibited c-fos transcription, failed to block the flow-induced PDGF expression, suggesting that flow-induced c-fos expression may not play an important role in the mechanism of flow-induced PDGF expression. The difference in the induction of c-fos and PDGF expression under pulsatile as compared to steady flow indicates that a complex, flow-mediated regulatory mechanism of gene expression exists in HUVEC. The increased expression of these proto-oncogenes mediated by flow may be important in regulating long-term cellular responses.
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| pubmed:grant | |
| pubmed:keyword | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0021-9541
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
154
|
| pubmed:geneSymbol |
c-fos
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
143-51
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:8419400-Base Sequence,
pubmed-meshheading:8419400-Blotting, Northern,
pubmed-meshheading:8419400-Cells, Cultured,
pubmed-meshheading:8419400-DNA, Single-Stranded,
pubmed-meshheading:8419400-Endothelium, Vascular,
pubmed-meshheading:8419400-Gene Expression Regulation,
pubmed-meshheading:8419400-Humans,
pubmed-meshheading:8419400-Kinetics,
pubmed-meshheading:8419400-Molecular Sequence Data,
pubmed-meshheading:8419400-Proto-Oncogene Proteins c-fos,
pubmed-meshheading:8419400-Pulsatile Flow,
pubmed-meshheading:8419400-Regional Blood Flow
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Pulsatile and steady flow induces c-fos expression in human endothelial cells.
|
| pubmed:affiliation |
Department of Chemical Engineering, Pennsylvania State University, University Park 16802.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
|