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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1993-2-8
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pubmed:abstractText |
A bias to either cell-mediated or antibody-mediated effector mechanisms is induced in an immune response against a pathogen, if activated T helper cells (Th) predominantly express Th1 [interleukin (IL)-2, interferon (IFN)-gamma, and tumor necrosis factor (TNF)-beta] or Th2 (IL-4, IL-5, IL-6 and IL-10) cytokines. Here we provide evidence that, due to the capability to secrete IL-1, macrophages, but not B cells, as antigen-presenting cells (APC) induce production of IFN-gamma in resting Th cells. Normal murine splenic Th cells were activated in vitro with the superantigen Staphylococcus aureus enterotoxin B (SEB) presented by macrophages as compared to other APC from murine spleen. As determined by immunofluorescence, Th cells producing IL-2 but almost none producing IL-4 and IL-5 are generated, irrespective of the type of APC. Generation of IFN-gamma-producing Th cells is largely dependent on presentation of SEB by macrophages. The requirement for macrophages, however, is overcome if IL-1 is provided. Expression of IFN-gamma by Th cells is not induced, if production of IL-1 by macrophages is inhibited by IL-10. Our results suggest a functional dichotomy of APC: normal resting Th cells differentiate into IL-2 and IFN-gamma secreting cells (Th1 cells) if antigen is presented by macrophages, whereas presentation by B cells generates Th cells secreting IL-2, which might differentiate into Th2 cells upon re-stimulation.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-10,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-2
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0014-2980
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
23
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
191-9
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:8419171-Animals,
pubmed-meshheading:8419171-Antigen-Presenting Cells,
pubmed-meshheading:8419171-Cells, Cultured,
pubmed-meshheading:8419171-Cytokines,
pubmed-meshheading:8419171-Fluorescent Antibody Technique,
pubmed-meshheading:8419171-Interferon-gamma,
pubmed-meshheading:8419171-Interleukin-1,
pubmed-meshheading:8419171-Interleukin-10,
pubmed-meshheading:8419171-Interleukin-2,
pubmed-meshheading:8419171-Macrophages,
pubmed-meshheading:8419171-Mice,
pubmed-meshheading:8419171-Mice, Inbred BALB C,
pubmed-meshheading:8419171-T-Lymphocytes, Helper-Inducer
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pubmed:year |
1993
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pubmed:articleTitle |
Regulation of T helper cell cytokine expression: functional dichotomy of antigen-presenting cells.
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pubmed:affiliation |
Institute for Genetics, University of Cologne, FRG.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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