|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
1993-1-29
|
|
pubmed:abstractText |
The gene encoding a 19-kDa antigen from Mycobacterium tuberculosis was expressed as a recombinant protein in the rapid-growing species Mycobacterium smegmatis. The recombinant antigen was expressed at a level approximately ninefold higher than in M. tuberculosis and, like the native antigen, was found in the pellet fraction after high-speed centrifugation of bacterial extracts. The 19-kDa antigen in crude bacterial extracts, and the purified recombinant antigen, bound strongly to concanavalin A, indicating the possibility of posttranslational glycosylation. The recombinant antigen stimulated T-cell proliferation in vitro when added to assays either in the form of whole recombinant bacteria or as a purified protein. Homologous expression of mycobacterial antigens in a rapid-growing mycobacterial host may be particularly useful for the immunological characterization of proteins which are subject to posttranslational modification.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8418047-1445568,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8418047-1545700,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8418047-1560200,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8418047-1692812,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8418047-1713602,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8418047-1717575,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8418047-1718849,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8418047-1900061,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8418047-1904126,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8418047-1904554,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8418047-1906192,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8418047-2046750,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8418047-2082148,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8418047-2158981,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8418047-2404875,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8418047-2466869,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8418047-2478323,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8418047-2493628,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8418047-2503277,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8418047-2503672,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8418047-2586628,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8418047-2673008,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8418047-2842799,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8418047-3065951,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8418047-3473289,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8418047-3553003,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8418047-388439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8418047-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8418047-6166820,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8418047-6191891
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jan
|
|
pubmed:issn |
0019-9567
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
61
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
260-7
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:8418047-Amino Acid Sequence,
pubmed-meshheading:8418047-Animals,
pubmed-meshheading:8418047-Antigens, Bacterial,
pubmed-meshheading:8418047-Blotting, Western,
pubmed-meshheading:8418047-Concanavalin A,
pubmed-meshheading:8418047-Dose-Response Relationship, Immunologic,
pubmed-meshheading:8418047-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:8418047-Glycosylation,
pubmed-meshheading:8418047-Lymphocyte Activation,
pubmed-meshheading:8418047-Mice,
pubmed-meshheading:8418047-Mice, Inbred C57BL,
pubmed-meshheading:8418047-Molecular Sequence Data,
pubmed-meshheading:8418047-Mycobacterium,
pubmed-meshheading:8418047-Mycobacterium tuberculosis,
pubmed-meshheading:8418047-Protein Processing, Post-Translational,
pubmed-meshheading:8418047-Recombinant Proteins,
pubmed-meshheading:8418047-T-Lymphocytes
|
|
pubmed:year |
1993
|
|
pubmed:articleTitle |
Expression of the Mycobacterium tuberculosis 19-kilodalton antigen in Mycobacterium smegmatis: immunological analysis and evidence of glycosylation.
|
|
pubmed:affiliation |
Medical Research Council Tuberculosis and Related Infections Unit, Royal Postgraduate Medical School, Hammersmith Hospital, London, United Kingdom.
|
|
pubmed:publicationType |
Journal Article
|
