Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
18
pubmed:dateCreated
1993-11-4
pubmed:abstractText
Transcriptional induction of genes in the lactose-galactose regulon of the yeast Kluyveromyces lactis requires the GAL4 transcription activator protein. Previous data indicated that the concentration of GAL4 was tightly regulated under basal, inducing, and glucose repressing conditions but the mechanisms were unknown. In this paper we demonstrate that transcription of the GAL4 gene (KI-GAL4) increases 3- to 4-fold during induction of the regulon. This increase requires a KI-GAL4 binding site, UASG, in front of the KI-GAL4 gene, indicating that the KI-GAL4 protein autoregulates transcription of its own gene. Our data demonstrate that the autoregulatory circuit is essential for full induction of the lactose-galactose regulon and, hence, for rapid growth on lactose or galactose. Other data indicate that basal transcription of the KI-GAL4 gene is governed by unidentified promoter elements. The existence of the autoregulatory circuit reveals an important difference between the lactose-galactose regulon and its homologue in Saccharomyces cerevisiae, the melibiose-galactose regulon. This difference may have evolved in response to different selective pressures encountered by the two organisms.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8414996-1317309, http://linkedlifedata.com/resource/pubmed/commentcorrection/8414996-1406674, http://linkedlifedata.com/resource/pubmed/commentcorrection/8414996-1569929, http://linkedlifedata.com/resource/pubmed/commentcorrection/8414996-1922058, http://linkedlifedata.com/resource/pubmed/commentcorrection/8414996-1923818, http://linkedlifedata.com/resource/pubmed/commentcorrection/8414996-1923819, http://linkedlifedata.com/resource/pubmed/commentcorrection/8414996-1924319, http://linkedlifedata.com/resource/pubmed/commentcorrection/8414996-1961715, http://linkedlifedata.com/resource/pubmed/commentcorrection/8414996-2001790, http://linkedlifedata.com/resource/pubmed/commentcorrection/8414996-2005880, http://linkedlifedata.com/resource/pubmed/commentcorrection/8414996-2076551, http://linkedlifedata.com/resource/pubmed/commentcorrection/8414996-2107531, http://linkedlifedata.com/resource/pubmed/commentcorrection/8414996-2201897, http://linkedlifedata.com/resource/pubmed/commentcorrection/8414996-2501650, http://linkedlifedata.com/resource/pubmed/commentcorrection/8414996-2830492, http://linkedlifedata.com/resource/pubmed/commentcorrection/8414996-3104772, http://linkedlifedata.com/resource/pubmed/commentcorrection/8414996-3125422, http://linkedlifedata.com/resource/pubmed/commentcorrection/8414996-3169576, http://linkedlifedata.com/resource/pubmed/commentcorrection/8414996-3550430, http://linkedlifedata.com/resource/pubmed/commentcorrection/8414996-390403, http://linkedlifedata.com/resource/pubmed/commentcorrection/8414996-6310321, http://linkedlifedata.com/resource/pubmed/commentcorrection/8414996-6394957, http://linkedlifedata.com/resource/pubmed/commentcorrection/8414996-6810093, http://linkedlifedata.com/resource/pubmed/commentcorrection/8414996-6853445, http://linkedlifedata.com/resource/pubmed/commentcorrection/8414996-8474461
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0305-1048
pubmed:author
pubmed:issnType
Print
pubmed:day
11
pubmed:volume
21
pubmed:geneSymbol
Kl-GAL4, URA3
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4378-82
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Autoregulation of GAL4 transcription is essential for rapid growth of Kluyveromyces lactis on lactose and galactose.
pubmed:affiliation
Department of Biochemistry, University of Kentucky, Lexington 40536-0084.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't