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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
1993-11-18
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pubmed:databankReference |
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pubmed:abstractText |
We studied the process by which whd, a P-element insertion allele of the Drosophila melanogaster white locus, is replaced by its homolog in the presence of transposase. These events are interpreted as the result of double-strand gap repair following excision of the P transposon in whd. We used a series of alleles derived from whd through P-element mobility as templates for this repair. One group of alleles, referred to collectively as whd-F, carried fragments of the P element that had lost some of the sequences needed in cis for mobility. The other group, whd-D, had lost all of the P insert and had some of the flanking DNA from white deleted. The average replacement frequencies were 43% for whd-F alleles and 7% for the whd-D alleles. Some of the former were converted at frequencies exceeding 50%. Our data suggest that the high conversion frequencies for the whd-F templates can be attributed at least in part to an elevated efficiency of repair of unexpanded gaps that is possibly caused by the closer match between whd-F sequences and the unexpanded gap endpoints. In addition, we found that the gene substitutions were almost exclusively in the direction of whd being replaced by the whd-F or whd-D allele rather than the reverse. The template alleles were usually unaltered in the process. This asymmetry implies that the conversion process is unidirectional and that the P fragments are not good substrates for P-element transposase. Our results help elucidate a highly efficient double-strand gap repair mechanism in D. melanogaster that can also be used for gene replacement procedures involving insertions and deletions. They also help explain the rapid spread of P elements in populations.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8413290-1285420,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8413290-1311850,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8413290-1313147,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8413290-1313335,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8413290-1321331,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8413290-1323316,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8413290-1325388,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8413290-1335623,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8413290-1411547,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8413290-1505030,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8413290-1571289,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8413290-1620105,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8413290-1653452,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8413290-1653453,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8413290-1732731,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8413290-1740110,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8413290-1846960,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8413290-1850084,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8413290-2155157,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8413290-2165865,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8413290-2545527,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8413290-2553268,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8413290-2835286,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8413290-3007275,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8413290-3016507,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8413290-3021567,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8413290-3025586,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8413290-3037544,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8413290-3043177,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8413290-3161076,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8413290-3174659,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8413290-3557108,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/8413290-553801,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8413290-6096689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8413290-6295640,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8413290-6297750,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8413290-6300863,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8413290-6309410,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8413290-6380756,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8413290-6941271,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8413290-8381961,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8413290-8384146,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8413290-940351
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0270-7306
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
13
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pubmed:geneSymbol |
w<up>hd-D</up>,
w<up>hd-F</up>,
w<up>hd</up>
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7006-18
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8413290-Alleles,
pubmed-meshheading:8413290-Animals,
pubmed-meshheading:8413290-Base Sequence,
pubmed-meshheading:8413290-Crosses, Genetic,
pubmed-meshheading:8413290-Crossing Over, Genetic,
pubmed-meshheading:8413290-DNA,
pubmed-meshheading:8413290-DNA Primers,
pubmed-meshheading:8413290-DNA Transposable Elements,
pubmed-meshheading:8413290-Drosophila melanogaster,
pubmed-meshheading:8413290-Eye Color,
pubmed-meshheading:8413290-Female,
pubmed-meshheading:8413290-Gene Conversion,
pubmed-meshheading:8413290-Gene Deletion,
pubmed-meshheading:8413290-Male,
pubmed-meshheading:8413290-Models, Genetic,
pubmed-meshheading:8413290-Molecular Sequence Data,
pubmed-meshheading:8413290-Polymerase Chain Reaction,
pubmed-meshheading:8413290-Templates, Genetic
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pubmed:year |
1993
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pubmed:articleTitle |
P-element-induced interallelic gene conversion of insertions and deletions in Drosophila melanogaster.
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pubmed:affiliation |
Genetics Department, University of Wisconsin, Madison 53706.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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