Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
10
|
| pubmed:dateCreated |
1993-11-10
|
| pubmed:abstractText |
A new human pre-B acute lymphoblastic leukemia cell line (KMO-90) was established from the bone marrow sample of a 12-year-old girl with acute lymphoblastic leukemia (ALL) carrying 1;19 chromosome translocation. KMO-90 cells expressed HLA-DR, CD10, CD19, and CD22 antigens. These cells had also cytoplasmic immunoglobulin lacking surface immunoglobulin, indicating that these had a pre-B phenotype. Chromosome analysis of this cell line showed 48, XX, +8, +19, t(1;19)(q23;p13). Southern blot analysis showed the same sized rearrangements of the E2A gene in KMO-90 cells as those in the original leukemic cells. By means of reverse transcriptase-polymerase chain reaction analysis, we detected E2A/PBX1 fusion transcripts in KMO-90 cells. KMO-90 is useful when studying the role of the 1;19 translocation in the etiology of pre-B ALL. Furthermore, we studied alterations of the p53 gene in this cell line by polymerase chain reaction, single-strand conformation polymorphism analysis. KMO-90 cells were identified to have a point mutation at codon 177 (CCC-->TCC) of the p53 gene, suggesting that alterations of the p53 gene may have an important role in the establishment of this cell line.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0887-6924
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
7
|
| pubmed:geneSymbol |
E2A
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1615-20
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8412323-Antigens, Surface,
pubmed-meshheading:8412323-Base Sequence,
pubmed-meshheading:8412323-Blotting, Southern,
pubmed-meshheading:8412323-Child,
pubmed-meshheading:8412323-Chromosomes, Human, Pair 1,
pubmed-meshheading:8412323-Chromosomes, Human, Pair 19,
pubmed-meshheading:8412323-DNA, Neoplasm,
pubmed-meshheading:8412323-DNA, Single-Stranded,
pubmed-meshheading:8412323-Female,
pubmed-meshheading:8412323-Genes, p53,
pubmed-meshheading:8412323-Humans,
pubmed-meshheading:8412323-Karyotyping,
pubmed-meshheading:8412323-Molecular Sequence Data,
pubmed-meshheading:8412323-Nucleic Acid Conformation,
pubmed-meshheading:8412323-Polymerase Chain Reaction,
pubmed-meshheading:8412323-Polymorphism, Genetic,
pubmed-meshheading:8412323-Precursor B-Cell Lymphoblastic Leukemia-Lymphoma,
pubmed-meshheading:8412323-Precursor Cell Lymphoblastic Leukemia-Lymphoma,
pubmed-meshheading:8412323-Translocation, Genetic,
pubmed-meshheading:8412323-Tumor Cells, Cultured
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Establishment of a new human pre-B acute lymphoblastic leukemia cell line (KMO-90) with 1;19 translocation carrying p53 gene alterations.
|
| pubmed:affiliation |
Department of Pediatrics, Gunma University School of Medicine, Maebashi, Japan.
|
| pubmed:publicationType |
Journal Article,
Case Reports,
Research Support, Non-U.S. Gov't
|