Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1993-11-24
pubmed:abstractText
The upstream regulatory region (URR) of human papillomavirus type 18 (HPV-18) harbors transcriptional promoter and enhancer elements which are thought to determine the cell-type specificity of the virus. In order to study the regulation of HPV-18 expression in vivo, we constructed transgenic mice carrying the bacterial lacZ gene under the control of the HPV-18 URR. Analysis of beta-galactosidase activity by histochemical staining of tissue sections of four independent transgenic mice showed that the viral promoter was specifically active in epithelial cells within a variety of organs (e.g., tongue, ovary, uterus, testis, and small intestine). Very strong staining was observed in newborn transgenic mice in contrast to a weak activity found during fetal life. Determination of beta-galactosidase activity in crude extracts from tissues of three lines of transgenic mice proved to be a useful tool for a quantitative analysis of transgene expression. In mice from two different transgenic lines treated with dexamethasone such measurements revealed a biphasic effect of the hormone on the activity of the enzyme in the stratified epithelium of the tongue (transient increase followed by a decrease). Northern (RNA) blot analysis showed similar changes in beta-galactosidase mRNA in that tissue. Treatment with tetradecanoyl phorbol acetate (TPA) led to a twofold increase in both enzymatic activity and mRNA levels. Finally, combined treatments with dexamethasone and TPA showed that both factors interfered with each other in their respective effects on transgene expression, suggesting that a cross-talk mechanism between transcription factors could be involved in the regulation of the HPV-18 URR.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8411377-1310950, http://linkedlifedata.com/resource/pubmed/commentcorrection/8411377-1314463, http://linkedlifedata.com/resource/pubmed/commentcorrection/8411377-1316480, http://linkedlifedata.com/resource/pubmed/commentcorrection/8411377-1318198, http://linkedlifedata.com/resource/pubmed/commentcorrection/8411377-1330541, http://linkedlifedata.com/resource/pubmed/commentcorrection/8411377-1645869, http://linkedlifedata.com/resource/pubmed/commentcorrection/8411377-1654457, http://linkedlifedata.com/resource/pubmed/commentcorrection/8411377-1656460, http://linkedlifedata.com/resource/pubmed/commentcorrection/8411377-1659743, http://linkedlifedata.com/resource/pubmed/commentcorrection/8411377-1668190, http://linkedlifedata.com/resource/pubmed/commentcorrection/8411377-1751545, http://linkedlifedata.com/resource/pubmed/commentcorrection/8411377-1847520, http://linkedlifedata.com/resource/pubmed/commentcorrection/8411377-1943996, http://linkedlifedata.com/resource/pubmed/commentcorrection/8411377-2155400, http://linkedlifedata.com/resource/pubmed/commentcorrection/8411377-2169351, http://linkedlifedata.com/resource/pubmed/commentcorrection/8411377-2169352, http://linkedlifedata.com/resource/pubmed/commentcorrection/8411377-2169353, http://linkedlifedata.com/resource/pubmed/commentcorrection/8411377-2170687, http://linkedlifedata.com/resource/pubmed/commentcorrection/8411377-2317863, http://linkedlifedata.com/resource/pubmed/commentcorrection/8411377-2434329, http://linkedlifedata.com/resource/pubmed/commentcorrection/8411377-2453058, http://linkedlifedata.com/resource/pubmed/commentcorrection/8411377-2476572, http://linkedlifedata.com/resource/pubmed/commentcorrection/8411377-2536832, http://linkedlifedata.com/resource/pubmed/commentcorrection/8411377-2545902, http://linkedlifedata.com/resource/pubmed/commentcorrection/8411377-2552162, http://linkedlifedata.com/resource/pubmed/commentcorrection/8411377-2822969, http://linkedlifedata.com/resource/pubmed/commentcorrection/8411377-2828035, http://linkedlifedata.com/resource/pubmed/commentcorrection/8411377-2845145, http://linkedlifedata.com/resource/pubmed/commentcorrection/8411377-2848240, http://linkedlifedata.com/resource/pubmed/commentcorrection/8411377-2875241, http://linkedlifedata.com/resource/pubmed/commentcorrection/8411377-2983187, http://linkedlifedata.com/resource/pubmed/commentcorrection/8411377-3018579, http://linkedlifedata.com/resource/pubmed/commentcorrection/8411377-3023067, http://linkedlifedata.com/resource/pubmed/commentcorrection/8411377-3029703, http://linkedlifedata.com/resource/pubmed/commentcorrection/8411377-3038518, http://linkedlifedata.com/resource/pubmed/commentcorrection/8411377-3099292, http://linkedlifedata.com/resource/pubmed/commentcorrection/8411377-3102226, http://linkedlifedata.com/resource/pubmed/commentcorrection/8411377-3133553, http://linkedlifedata.com/resource/pubmed/commentcorrection/8411377-3246353, http://linkedlifedata.com/resource/pubmed/commentcorrection/8411377-6093261, http://linkedlifedata.com/resource/pubmed/commentcorrection/8411377-6311083, http://linkedlifedata.com/resource/pubmed/commentcorrection/8411377-6312838, http://linkedlifedata.com/resource/pubmed/commentcorrection/8411377-6329740, http://linkedlifedata.com/resource/pubmed/commentcorrection/8411377-6418386, http://linkedlifedata.com/resource/pubmed/commentcorrection/8411377-6960240, http://linkedlifedata.com/resource/pubmed/commentcorrection/8411377-8388016, http://linkedlifedata.com/resource/pubmed/commentcorrection/8411377-911786, http://linkedlifedata.com/resource/pubmed/commentcorrection/8411377-942051
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
67
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6742-52
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:8411377-Animals, pubmed-meshheading:8411377-Animals, Newborn, pubmed-meshheading:8411377-Base Sequence, pubmed-meshheading:8411377-Enhancer Elements, Genetic, pubmed-meshheading:8411377-Gene Expression Regulation, Viral, pubmed-meshheading:8411377-Genes, Reporter, pubmed-meshheading:8411377-Glucocorticoids, pubmed-meshheading:8411377-Mice, pubmed-meshheading:8411377-Mice, Transgenic, pubmed-meshheading:8411377-Molecular Sequence Data, pubmed-meshheading:8411377-Papillomaviridae, pubmed-meshheading:8411377-Promoter Regions, Genetic, pubmed-meshheading:8411377-Proto-Oncogene Proteins c-jun, pubmed-meshheading:8411377-Receptors, Glucocorticoid, pubmed-meshheading:8411377-Restriction Mapping, pubmed-meshheading:8411377-Skin, pubmed-meshheading:8411377-Tetradecanoylphorbol Acetate
pubmed:year
1993
More...