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rdf:type |
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lifeskim:mentions |
umls-concept:C0019682,
umls-concept:C0020291,
umls-concept:C0026473,
umls-concept:C0039194,
umls-concept:C0042774,
umls-concept:C0079904,
umls-concept:C0086418,
umls-concept:C0442805,
umls-concept:C1515877,
umls-concept:C1879547,
umls-concept:C1959616
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pubmed:issue |
11
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pubmed:dateCreated |
1993-11-24
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pubmed:abstractText |
We have tested whether breakdown of phosphatidylcholine (PC) initiated by exogenous addition of a PC-specific phospholipase C (PC-PLC) from Bacillus cereus or by endogenous overexpression of PC-PLC induces functional activation of NF-kappa B and increases human immunodeficiency virus (HIV) enhancer activity. PC-PLC-activated hydrolysis of PC was found to induce bona fide p50/p65 NF-kappa B binding activity in three different cell lines of human or murine origin. No significant changes in the turnover of other cellular phospholipids were detected in PC-PLC-treated cells. Induction of NF-kappa B by PC-PLC did not depend on de novo synthesis of proteins or autocrine secretion of either tumor necrosis factor or interleukin 1. In human monocytic and lymphoblastoid T-cell lines, induction of NF-kappa B by PC-PLC resulted in clear induction of luciferase expression vectors placed under the control of synthetic kappa B enhancers or wild type, but not kappa B-mutated, HIV long terminal repeat constructs. HIV replication was increased by PC-PLC in chronically infected monocytes and T lymphocytes. NF-kappa B activation promoted by addition of exogenous PC-PLC correlated with an intense production of diacylglycerol. However, addition of a phosphatidylinositol-specific PLC from B. cereus also induced diacylglycerol but did not activate kappa B enhancer-directed vectors. PC-PLC-induced NF-kappa B activation could not be blocked by a specific inhibitor of phorbol ester-inducible protein kinases C. These results indicate that a cellular transduction pathway, dependent on specific PC breakdown, is functional in T lymphocytes and monocytes and may be used by various transmembrane receptors to activate HIV transcription through NF-kappa B-dependent induction of the HIV enhancer.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8411362-1455508,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8411362-1502202,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8411362-1639070,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8411362-1658188,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8411362-1839358,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8411362-1845988,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8411362-1874734,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8411362-2006423,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8411362-2023633,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8411362-2023953,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8411362-2104616,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8411362-2112426,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8411362-2123453,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8411362-2146676,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8411362-2157987,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8411362-2186905,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8411362-2203531,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8411362-2315321,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8411362-2470089,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8411362-2477457,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8411362-2506180,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8411362-2574206,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8411362-2576770,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8411362-2682462,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8411362-2825351,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8411362-2898301,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8411362-3031512,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8411362-3140380,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8411362-3313065,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8411362-3462727,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8411362-3510743,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8411362-6270351,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8411362-8336714,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8411362-8423794,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8411362-8424175
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
67
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6596-604
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8411362-Cell Line,
pubmed-meshheading:8411362-Enhancer Elements, Genetic,
pubmed-meshheading:8411362-Gene Expression Regulation, Viral,
pubmed-meshheading:8411362-HIV Long Terminal Repeat,
pubmed-meshheading:8411362-HIV-1,
pubmed-meshheading:8411362-Humans,
pubmed-meshheading:8411362-Monocytes,
pubmed-meshheading:8411362-NF-kappa B,
pubmed-meshheading:8411362-Phosphatidylcholines,
pubmed-meshheading:8411362-Protein Kinase C,
pubmed-meshheading:8411362-Signal Transduction,
pubmed-meshheading:8411362-T-Lymphocytes,
pubmed-meshheading:8411362-Transcription, Genetic,
pubmed-meshheading:8411362-Type C Phospholipases,
pubmed-meshheading:8411362-Virus Replication
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pubmed:year |
1993
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pubmed:articleTitle |
Phosphatidylcholine hydrolysis activates NF-kappa B and increases human immunodeficiency virus replication in human monocytes and T lymphocytes.
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pubmed:affiliation |
Unite d'Immunologie Virale, Institut Pasteur, Paris, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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