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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1993-11-24
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pubmed:abstractText |
Leptomonas seymouri, a monogenetic trypanosomatid originally isolated from Dysdercus suturellus (Hemiptera), was used to develop a reverse genetic system for trypanosomatid flagellates. In many eukaryotic cell types, reverse genetics has proven to be a powerful tool for defining structure/function relationships within genes. The mini-exon genes of trypanosomatids encode key components of all cellular mRNAs. This component is a 5' "leader" RNA that is spliced onto all mRNA precursors during mRNA formation within the cell nucleus. The data presented here indicate that structure/function relationships within the mini-exon gene can be probed using the molecular genetic system developed and characterized for L. seymouri.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0022-3395
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
79
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
637-44
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:8410533-Animals,
pubmed-meshheading:8410533-Blotting, Northern,
pubmed-meshheading:8410533-Cell Line, Transformed,
pubmed-meshheading:8410533-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:8410533-Cloning, Molecular,
pubmed-meshheading:8410533-Culture Media,
pubmed-meshheading:8410533-DNA, Protozoan,
pubmed-meshheading:8410533-DNA Replication,
pubmed-meshheading:8410533-Drug Resistance,
pubmed-meshheading:8410533-Electrophoresis, Agar Gel,
pubmed-meshheading:8410533-Electroporation,
pubmed-meshheading:8410533-Exons,
pubmed-meshheading:8410533-Gene Expression Regulation,
pubmed-meshheading:8410533-Genetic Vectors,
pubmed-meshheading:8410533-Models, Biological,
pubmed-meshheading:8410533-Plasmids,
pubmed-meshheading:8410533-RNA, Messenger,
pubmed-meshheading:8410533-RNA Processing, Post-Transcriptional,
pubmed-meshheading:8410533-Transcription, Genetic,
pubmed-meshheading:8410533-Transfection,
pubmed-meshheading:8410533-Transformation, Genetic,
pubmed-meshheading:8410533-Trypanosomatina,
pubmed-meshheading:8410533-Tubulin
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pubmed:year |
1993
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pubmed:articleTitle |
Leptomonas seymouri as a model system for the analysis of gene expression in trypanosomatids.
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pubmed:affiliation |
Laboratory of Molecular Parasitology, Rockefeller University, New York, New York 10021.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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