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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1993-11-12
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pubmed:abstractText |
Prior studies have suggested that protein kinase A (PKA)-mediated inhibition of the rabbit renal brush border membrane (BBM) Na(+)-H+ exchanger involves a regulatory protein that is distinct from the transporter. This putative regulatory protein was purified by column chromatography and SDS-PAGE, and a partial primary amino acid sequence was determined. An affinity-purified polyclonal antibody to a synthetic peptide representing a sequence of the protein recognized a polypeptide of 55 kD in BBM but not in basolateral membrane. The antibody immunoprecipitated a PKA substrate of a similar molecular mass from detergent-solubilized BBM proteins. When assayed after reconstitution, PKA in the presence of ATP and Mg2+ did not inhibit Na(+)-H+ exchange transport in a fraction of solubilized BBM proteins eluting from an anion exchange column between 0.2 and 0.4 M NaCl (fraction B). Coreconstitution of fraction B with the immunoprecipitated 55-kD protein restored the inhibitory effect of PKA (change = 42%, P < 0.05). By contrast, Na(+)-H+ exchange transport in total solubilized BBM proteins was inhibited 25% (P < 0.05) by PKA, ATP, and Mg2+. This effect was abolished by immunodepletion of the cAMP regulatory protein (change = +5%, P = NS). These findings provide evidence that the regulation of renal BBM Na(+)-H+ exchange transport by PKA is affected by repletion and depletion of a specific protein. This suggests that PKA-mediated inhibition of the renal BBM Na(+)-H+ exchanger requires participation of a regulatory protein that is distinct from the transporter itself.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8408631-1328833,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8408631-1374392,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8408631-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8408631-2159717,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8408631-2175560,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8408631-217761,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8408631-2552124,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8408631-2552817,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8408631-2835485,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8408631-2982285,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8408631-2994494,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8408631-3028154,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8408631-3523137,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8408631-388439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8408631-4375763,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8408631-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8408631-6548944,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8408631-6955305
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0021-9738
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
92
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1781-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8408631-Amino Acid Sequence,
pubmed-meshheading:8408631-Animals,
pubmed-meshheading:8408631-Blotting, Western,
pubmed-meshheading:8408631-Cyclic AMP,
pubmed-meshheading:8408631-Cyclic AMP-Dependent Protein Kinases,
pubmed-meshheading:8408631-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:8408631-Kidney,
pubmed-meshheading:8408631-Liposomes,
pubmed-meshheading:8408631-Membrane Proteins,
pubmed-meshheading:8408631-Microvilli,
pubmed-meshheading:8408631-Molecular Sequence Data,
pubmed-meshheading:8408631-Molecular Weight,
pubmed-meshheading:8408631-Phosphoproteins,
pubmed-meshheading:8408631-Proteolipids,
pubmed-meshheading:8408631-Rabbits,
pubmed-meshheading:8408631-Sequence Homology, Amino Acid,
pubmed-meshheading:8408631-Sodium-Hydrogen Antiporter,
pubmed-meshheading:8408631-Transducin
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pubmed:year |
1993
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pubmed:articleTitle |
CAMP-mediated inhibition of the renal brush border membrane Na+-H+ exchanger requires a dissociable phosphoprotein cofactor.
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pubmed:affiliation |
Department of Medicine, University of California, Los Angeles School of Medicine.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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