|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
9
|
|
pubmed:dateCreated |
1993-11-2
|
|
pubmed:abstractText |
We used the polymerase chain reaction and primers corresponding to three regions of the human cytomegalovirus (HCMV) genome to study HCMVs isolated from 16 children attending a single day-care center and the father of two children in the same center. When we analyzed isolates with primers for the pp65 and major immediate-early genes, we observed nearly uniform amplification yielding products of predicted sizes. By contrast, primers for the a sequence demonstrated variability among HCMV strains, supporting the use of these primers as an epidemiologic tool. Analysis of a-sequence products from two isolates demonstrated 50 to 70% nucleotide homology with the a sequence of HCMV Towne strain DNA. We observed 95% nucleotide homology for the two a-sequence products derived from the father-child pair. Analysis of day-care center isolates indicated that two children excreted two distinct HCMV strains during the study interval.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8408568-1315336,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8408568-1328286,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8408568-1653938,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8408568-1663509,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8408568-1709960,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8408568-1980680,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8408568-2552148,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8408568-2831327,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8408568-2995501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8408568-3014103,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8408568-3016115,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8408568-3016322,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8408568-3029241,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8408568-3039048,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8408568-6094679
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Sep
|
|
pubmed:issn |
0095-1137
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
31
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
2433-8
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:8408568-Base Sequence,
pubmed-meshheading:8408568-Cytomegalovirus,
pubmed-meshheading:8408568-Cytomegalovirus Infections,
pubmed-meshheading:8408568-DNA, Bacterial,
pubmed-meshheading:8408568-DNA Restriction Enzymes,
pubmed-meshheading:8408568-Humans,
pubmed-meshheading:8408568-Molecular Sequence Data,
pubmed-meshheading:8408568-Polymerase Chain Reaction,
pubmed-meshheading:8408568-Sequence Homology, Nucleic Acid
|
|
pubmed:year |
1993
|
|
pubmed:articleTitle |
Analysis of acquired human cytomegalovirus infections by polymerase chain reaction.
|
|
pubmed:affiliation |
Department of Pediatrics, University of Iowa College of Medicine, Iowa City 52242.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|
