rdf:type |
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lifeskim:mentions |
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pubmed:issue |
19
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pubmed:dateCreated |
1993-11-16
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pubmed:databankReference |
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pubmed:abstractText |
Degenerate oligonucleotide primers were used in a polymerase chain reaction (PCR) to amplify a region of the recA sequence of Streptococcus gordonii Challis. The resulting PCR fragment was cloned into the suicide vector pAM6199 and introduced into strain Challis, giving rise to recombination-deficient strains in which the recA gene was specifically inactivated.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8407809-1091546,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8407809-1195397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8407809-1409970,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8407809-1534326,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8407809-1556091,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8407809-1628842,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8407809-1945494,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8407809-2252387,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8407809-231270,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8407809-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8407809-3988349,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8407809-4202995,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8407809-5464272,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8407809-6279083,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8407809-6782083,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8407809-6946273,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8407809-6953305,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8407809-7399689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8407809-785000,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8407809-824275
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
175
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pubmed:geneSymbol |
recA
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6354-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8407809-Amino Acid Sequence,
pubmed-meshheading:8407809-Cloning, Molecular,
pubmed-meshheading:8407809-DNA Primers,
pubmed-meshheading:8407809-Deoxyribonuclease HindIII,
pubmed-meshheading:8407809-Escherichia coli,
pubmed-meshheading:8407809-Genes, Bacterial,
pubmed-meshheading:8407809-Molecular Sequence Data,
pubmed-meshheading:8407809-Mutagenesis, Site-Directed,
pubmed-meshheading:8407809-Plasmids,
pubmed-meshheading:8407809-Polymerase Chain Reaction,
pubmed-meshheading:8407809-Rec A Recombinases,
pubmed-meshheading:8407809-Recombination, Genetic,
pubmed-meshheading:8407809-Restriction Mapping,
pubmed-meshheading:8407809-Sequence Homology, Amino Acid,
pubmed-meshheading:8407809-Streptococcus,
pubmed-meshheading:8407809-Streptococcus mutans
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pubmed:year |
1993
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pubmed:articleTitle |
Construction of recombination-deficient strains of Streptococcus gordonii by disruption of the recA gene.
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pubmed:affiliation |
Department of Cariology, School of Dentistry, University of Michigan, Ann Arbor 48109.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
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