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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
8
|
| pubmed:dateCreated |
1993-11-17
|
| pubmed:abstractText |
During the latent period of murine erythroleukemia (MEL) cell differentiation, c-myc levels showed a significant change and the overexpression of the transferred c-myc gene inhibited the commitment and differentiation of MEL cells, suggesting that c-Myc may be a key molecule for the commitment. Since c-Myc may function as a DNA binding transcription factor, we examined whether c-Myc regulates the latent period genes (hsp and hsc70, MER5, Id and Spi-1 genes) and the erythroid-specific genes [beta-globin, glycophorin, delta-aminolevulinic acid synthase (ALAS-E), GATA-1 and erythropoietin receptor (EpoR)] in the MEL cell transformant having transferred c-myc gene. The overexpression of c-myc gene affected the latent period genes in different ways: hsc and hsp 70 genes and Id gene were positively regulated, while expression of MER5 gene was repressed. While c-myc is thought to be involved in DNA replication, its overexpression showed no effect on the expression of proliferating cell specific nuclear antigen or DNA polymerase a. The overexpression of c-myc repressed the expression of glycophorin, ALAS-E and beta-globin genes, of the five erythroid-specific genes, but had no effect on expression of GATA-1 or EpoR gene. These results suggest that c-Myc differentially regulates the expression of the latent period and erythroid-specific genes.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0910-5050
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
84
|
| pubmed:geneSymbol |
Id,
MER5
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
885-92
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:8407552-Animals,
pubmed-meshheading:8407552-Cell Line, Transformed,
pubmed-meshheading:8407552-Cell Transformation, Neoplastic,
pubmed-meshheading:8407552-Gene Expression Regulation,
pubmed-meshheading:8407552-Genes, myc,
pubmed-meshheading:8407552-Leukemia, Erythroblastic, Acute,
pubmed-meshheading:8407552-Mice,
pubmed-meshheading:8407552-Transcriptional Activation
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
c-Myc selectively regulates the latent period and erythroid-specific genes in murine erythroleukemia cell differentiation.
|
| pubmed:affiliation |
Department of Cell Biology, Tohoku University, Sendai.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|