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rdf:type |
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lifeskim:mentions |
umls-concept:C0021756,
umls-concept:C0039194,
umls-concept:C0205263,
umls-concept:C0330390,
umls-concept:C0337112,
umls-concept:C0439097,
umls-concept:C0443199,
umls-concept:C1123019,
umls-concept:C1524075,
umls-concept:C1547348,
umls-concept:C1552644,
umls-concept:C1705241,
umls-concept:C1823153,
umls-concept:C1880022,
umls-concept:C2003941,
umls-concept:C2349976
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pubmed:issue |
3
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pubmed:dateCreated |
1993-11-5
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pubmed:abstractText |
A combination of concanavalin A (Con A)-stimulated ovine lymph node (LN) cells and Chinese hamster ovarian (CHO) cells stably transfected with the ovine interleukin-2 receptor-alpha (IL-2R alpha) chain cDNA (CHO IL-2R cells) were used in a differential immunization strategy to generate several monoclonal antibodies (mAb) against the ovine IL-2R alpha chain. The specificity of one of the mAb, designated mAb 9-14, for the ovine IL-2R alpha chain was demonstrated by its reactivity with Con A-stimulated LN cells and CHO IL-2R cells, immunoprecipitation of a 47,000 MW protein from CHO IL-2R cells and inhibition of IL-2-dependent proliferation of Con A-stimulated ovine LN cells. Examination of IL-2R alpha chain expression on resting lamb peripheral blood lymphocyte populations showed a high frequency of IL-2R alpha chain expression on CD4 T cells but not on CD8 T cells, CD45RA+ cells or gamma delta T cells, which comprise up to 60% of lamb peripheral blood T cells. The kinetics of IL-2R alpha chain induction on Con A-stimulated peripheral blood alpha beta and gamma delta T cells was compared. A rapid induction of IL-2R alpha chain expression on precultured gamma delta T cells but not alpha beta T cells was observed within 6 hr of Con A stimulation. A preculturing period was required to 'prime' gamma delta T cells for rapid responsiveness to Con A. Using appropriate inhibitors, we demonstrated that both transcription and translation events were required for rapid IL-2R expression on precultured gamma delta T cells and therefore the 'priming' of gamma delta T cells by in vitro culture did not involve an accumulation of IL-2R alpha chain mRNA or preformed receptors within these cells.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8406574-1313950,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8406574-1346150,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8406574-1371469,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8406574-1628887,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8406574-1631559,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8406574-1685671,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8406574-1692080,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8406574-1695022,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8406574-1702138,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8406574-1831127,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8406574-1900055,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8406574-2005404,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8406574-2120339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8406574-2145167,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8406574-2258612,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8406574-2307933,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8406574-2445494,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8406574-2494663,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8406574-2497520,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8406574-2528206,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8406574-2528463,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8406574-2535142,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8406574-2544808,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8406574-2687377,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8406574-2783942,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8406574-2785134,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8406574-2785992,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8406574-2813410,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8406574-2836068,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8406574-2966101,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8406574-2996141,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8406574-3081353,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8406574-3116145,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8406574-3309626,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8406574-3930598,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8406574-5706694,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8406574-6090574,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8406574-6327813
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Concanavalin A,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-2
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0019-2805
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
79
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
471-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8406574-Animals,
pubmed-meshheading:8406574-Antibodies, Monoclonal,
pubmed-meshheading:8406574-Cells, Cultured,
pubmed-meshheading:8406574-Concanavalin A,
pubmed-meshheading:8406574-Female,
pubmed-meshheading:8406574-Lymphocyte Activation,
pubmed-meshheading:8406574-Mice,
pubmed-meshheading:8406574-Mice, Inbred BALB C,
pubmed-meshheading:8406574-Protein Biosynthesis,
pubmed-meshheading:8406574-Receptors, Antigen, T-Cell, alpha-beta,
pubmed-meshheading:8406574-Receptors, Antigen, T-Cell, gamma-delta,
pubmed-meshheading:8406574-Receptors, Interleukin-2,
pubmed-meshheading:8406574-Sheep,
pubmed-meshheading:8406574-T-Lymphocyte Subsets,
pubmed-meshheading:8406574-T-Lymphocytes,
pubmed-meshheading:8406574-Transcription, Genetic
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pubmed:year |
1993
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pubmed:articleTitle |
Characterization of the ovine interleukin-2 receptor-alpha chain: differential induction on precultured alpha beta and gamma delta T cells.
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pubmed:affiliation |
Centre for Animal Biotechnology, School of Veterinary Science, University of Melbourne, Parkville, Australia.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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