Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1993-10-28
|
| pubmed:abstractText |
In a linkage analysis of Pax-3 and splotch no recombinations were found in 117 backcross mice. Molecular analysis of Pax-3 in three alleles of splotch shows a number of significant alterations to the Pax-3 gene. In Sp/Sp embryos, cDNA PCR analysis reveals a shortened transcript in which exon 4 of Pax-3 is deleted due to mutation of the splice acceptor site of intron 3. In the Sp4H allele, the Pax-3 gene is deleted and in Spd embryos, Pax-3 expression is significantly lower than that in normal littermate embryos. The linkage analysis, shortened Pax-3 transcript in Sp, and deletion of Pax-3 in Sp4H described here, together with the previous report of an intragenic deletion in Pax-3 in Sp2H mice and the deletion of Pax-3 in Spr mice, provide strong evidence for the allelic identity of Pax-3 and Sp.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0888-7543
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
17
|
| pubmed:geneSymbol |
Pax-3,
Sp,
Sp<up>2H</up>,
Sp<up>d</up>,
Sp<up>r</up>
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
355-63
|
| pubmed:dateRevised |
2010-11-18
|
| pubmed:meshHeading |
pubmed-meshheading:8406486-Alleles,
pubmed-meshheading:8406486-Alternative Splicing,
pubmed-meshheading:8406486-Animals,
pubmed-meshheading:8406486-Base Sequence,
pubmed-meshheading:8406486-Chromosome Mapping,
pubmed-meshheading:8406486-Crosses, Genetic,
pubmed-meshheading:8406486-Female,
pubmed-meshheading:8406486-Gene Deletion,
pubmed-meshheading:8406486-Genetic Linkage,
pubmed-meshheading:8406486-Heterozygote,
pubmed-meshheading:8406486-Homozygote,
pubmed-meshheading:8406486-Male,
pubmed-meshheading:8406486-Mice,
pubmed-meshheading:8406486-Mice, Inbred C3H,
pubmed-meshheading:8406486-Mice, Neurologic Mutants,
pubmed-meshheading:8406486-Molecular Sequence Data,
pubmed-meshheading:8406486-Mutation,
pubmed-meshheading:8406486-Oligodeoxyribonucleotides,
pubmed-meshheading:8406486-Phenotype,
pubmed-meshheading:8406486-Polymerase Chain Reaction,
pubmed-meshheading:8406486-Transcription, Genetic
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Analysis of the Pax-3 gene in the mouse mutant splotch.
|
| pubmed:affiliation |
Department of Molecular Cell Biology, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|