Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
10
|
| pubmed:dateCreated |
1993-11-19
|
| pubmed:abstractText |
The liver-enriched transcription factor DBP is expressed with a stringent circadian rhythm. We present evidence that DBP is a regulator of the circadian expression of the rat gene encoding cholesterol 7 alpha hydroxylase (C7 alpha H), the rate-limiting enzyme in the conversion of cholesterol to bile acids. As with DBP, C7 alpha H mRNA reaches peak levels in the evening, and its cycling is independent of daily food and light cues. As predicted for a DBP target gene, the primary level of C7 alpha H circadian expression is at the transcriptional level. DBP can activate the C7 alpha H promoter in cotransfection assays through a cognate DNA site centered around -225. In nuclear extracts prepared by a novel method that, in contrast to conventional techniques, yields near-quantitative recovery of DBP and other non-histone proteins, the DNA site required for DBP activation is the predominant site of occupancy by nuclear factors on the C7 alpha H promoter. At this site, the predominant binding activity is an evening-specific complex of which DBP is a component. These data suggest that DBP may play an important role in cholesterol homeostasis through circadian transcriptional regulation of cholesterol 7 alpha hydroxylase.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cholesterol 7-alpha-Hydroxylase,
http://linkedlifedata.com/resource/pubmed/chemical/DBP protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0890-9369
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
7
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1871-84
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:8405996-Animals,
pubmed-meshheading:8405996-Base Sequence,
pubmed-meshheading:8405996-Cell Nucleus,
pubmed-meshheading:8405996-Cells, Cultured,
pubmed-meshheading:8405996-Cholesterol 7-alpha-Hydroxylase,
pubmed-meshheading:8405996-Circadian Rhythm,
pubmed-meshheading:8405996-Cloning, Molecular,
pubmed-meshheading:8405996-DNA Mutational Analysis,
pubmed-meshheading:8405996-DNA-Binding Proteins,
pubmed-meshheading:8405996-Gene Expression Regulation,
pubmed-meshheading:8405996-Genes, Reporter,
pubmed-meshheading:8405996-Homeostasis,
pubmed-meshheading:8405996-Leucine Zippers,
pubmed-meshheading:8405996-Liver,
pubmed-meshheading:8405996-Molecular Sequence Data,
pubmed-meshheading:8405996-Promoter Regions, Genetic,
pubmed-meshheading:8405996-RNA, Messenger,
pubmed-meshheading:8405996-Rats,
pubmed-meshheading:8405996-Tissue Distribution,
pubmed-meshheading:8405996-Transcription, Genetic,
pubmed-meshheading:8405996-Transcription Factors
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Circadian transcription of the cholesterol 7 alpha hydroxylase gene may involve the liver-enriched bZIP protein DBP.
|
| pubmed:affiliation |
Département de Biologie Moléculaire, Sciences II, Université de Genève, Switzerland.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|