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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1993-11-2
|
| pubmed:abstractText |
Mouse motoneurons were isolated from dissociated E15 mouse spinal cord and grown on polyornithine-coated round coverslips in a growth medium (DMEM/F12) supplemented with progesterone, trans-ferrin, selenium, horse serum and muscle extract. Astrocytes from newborn mouse neopallium were grown on rectangular coverslips. The motoneuron neurite growth was determined at day 8 of culture by counting, using the light microscope, the intersections produced by neurites radiating from the perikaryon placed centrally in a graticule eyepiece of concentric circles. The mean intersections for cultures without addition of astrocytes, insulin, insulin-like growth factor I (IGF-I) or nerve growth factor (NGF) was 12.6 +/- 0.8. When astrocytes on a separate coverslip were introduced from day 1, there was a small increase in neurite growth (16.3 +/- 0.9). The neurite growth was further increased significantly with the addition of insulin (27.3 +/- 1.4), IGF-I (31.5 +/- 1.4) or NGF (21.8 +/- 1.1) to cultures with astrocytes. Insulin, IGF-I or NGF in the absence of astrocytes did not greatly increase the neurite growth. We conclude that insulin, IGF-I and NGF promote neurite growth through some interactions with astrocytes.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0165-3806
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
16
|
| pubmed:volume |
74
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
83-8
|
| pubmed:dateRevised |
2011-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:8403378-Animals,
pubmed-meshheading:8403378-Astrocytes,
pubmed-meshheading:8403378-Cells, Cultured,
pubmed-meshheading:8403378-Insulin,
pubmed-meshheading:8403378-Insulin-Like Growth Factor I,
pubmed-meshheading:8403378-Mice,
pubmed-meshheading:8403378-Motor Neurons,
pubmed-meshheading:8403378-Nerve Growth Factors,
pubmed-meshheading:8403378-Neurites,
pubmed-meshheading:8403378-Spinal Cord
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Neurite promoting activity of insulin, insulin-like growth factor I and nerve growth factor on spinal motoneurons is astrocyte dependent.
|
| pubmed:affiliation |
Department of Pathology (Neuropathology), University of Saskatchewan, Saskatoon, Canada.
|
| pubmed:publicationType |
Journal Article
|