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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
20
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pubmed:dateCreated |
1993-11-9
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pubmed:abstractText |
Mucins are highly immunogenic glycoproteins that are abundantly expressed by breast carcinomas and other carcinomas. The fact that deglycosylated normal mucin can induce tumor-specific monoclonal antibodies indicates that tumor-specific epitopes are hidden in the fully glycosylated form. Using recombinant DNA techniques, a fragment of mucin tandem repeats was inserted into pMal-p, an Escherichia coli expression vector, and resulted in the expression of an unglycosylated maltose-binding protein-mucin fusion protein. This fusion protein has been purified and showed strong affinity to breast tumor-specific monoclonal antibody SM3. The antisera against this recombinant mucin fusion protein recognized all breast tumor cell lines we tested. Competition assay with monoclonal antibody SM3 shows that anti-recombinant mucin fusion protein binds the epitope that SM3 binds. These results confirm the hypothesis that unglycosylated mucin contains a tumor-specific epitope. This leads to the possibility that recombinant mucin may be used to develop vaccines against breast cancer and cytotoxic T-lymphocyte lines for immunotherapy of breast cancer.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0008-5472
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
53
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4920-6
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:8402682-ATP-Binding Cassette Transporters,
pubmed-meshheading:8402682-Breast Neoplasms,
pubmed-meshheading:8402682-Carrier Proteins,
pubmed-meshheading:8402682-Cell Line,
pubmed-meshheading:8402682-Cloning, Molecular,
pubmed-meshheading:8402682-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:8402682-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:8402682-Escherichia coli,
pubmed-meshheading:8402682-Escherichia coli Proteins,
pubmed-meshheading:8402682-Female,
pubmed-meshheading:8402682-Fluorescent Antibody Technique,
pubmed-meshheading:8402682-Humans,
pubmed-meshheading:8402682-Luminescent Measurements,
pubmed-meshheading:8402682-Maltose-Binding Proteins,
pubmed-meshheading:8402682-Molecular Weight,
pubmed-meshheading:8402682-Monosaccharide Transport Proteins,
pubmed-meshheading:8402682-Mucins,
pubmed-meshheading:8402682-Recombinant Fusion Proteins,
pubmed-meshheading:8402682-Tumor Cells, Cultured
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pubmed:year |
1993
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pubmed:articleTitle |
Expression of a recombinant breast tumor-associated mucin fusion protein in Escherichia coli exposes the tumor-specific epitope.
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pubmed:affiliation |
Department of Internal Medicine, Texas Tech University Health Sciences Center, Amarillo.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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