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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1993-11-10
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pubmed:abstractText |
The aim of this study was to test the in vitro cytokine production by peripheral blood mononuclear cells (PBMCs) in patients with Fanconi's anemia (FA). Phytohemagglutinin (PHA)-stimulated PBMCs from 21 patients with FA were studied for their ability to produce interleukin 6 (IL-6), leukemia inhibitory factor (LIF) and granulocyte-macrophage colony stimulating factor (GM-CSF). Enzymatic immunoassay (EIA) was used for both IL-6 and LIF, while GM-CSF was evaluated in a highly sensitive biological assay provided by GM-CSF-dependent M-07e cells. A significant decrease of IL-6 was detected in 9 out of 11 FA patients compared with five normal donors, while similar amounts of LIF were produced from 21 FA patients and 21 healthy subjects. A drastic increase of active GM-CSF was documented in PHA-stimulated PBMC-conditioned medium in all 18 FA patients tested. Since IL-6 and GM-CSF play an important role in maintaining basal hemopoiesis, our results suggest that an abnormal cytokine network may be involved in the pathogenesis of FA pancytopenia.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1066-5099
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
11 Suppl 2
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pubmed:owner |
NLM
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pubmed:authorsComplete |
N
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pubmed:pagination |
137-43
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8401257-Adolescent,
pubmed-meshheading:8401257-Adult,
pubmed-meshheading:8401257-Bone Marrow,
pubmed-meshheading:8401257-Cells, Cultured,
pubmed-meshheading:8401257-Child,
pubmed-meshheading:8401257-Child, Preschool,
pubmed-meshheading:8401257-Culture Media, Conditioned,
pubmed-meshheading:8401257-Fanconi Anemia,
pubmed-meshheading:8401257-Female,
pubmed-meshheading:8401257-Fibroblasts,
pubmed-meshheading:8401257-Granulocyte-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:8401257-Growth Inhibitors,
pubmed-meshheading:8401257-Humans,
pubmed-meshheading:8401257-Interleukin-6,
pubmed-meshheading:8401257-Leukemia Inhibitory Factor,
pubmed-meshheading:8401257-Leukocytes, Mononuclear,
pubmed-meshheading:8401257-Lymphocyte Activation,
pubmed-meshheading:8401257-Lymphokines,
pubmed-meshheading:8401257-Male,
pubmed-meshheading:8401257-Phytohemagglutinins
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pubmed:year |
1993
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pubmed:articleTitle |
Production of interleukin 6, leukemia inhibitory factor and granulocyte-macrophage colony stimulating factor by peripheral blood mononuclear cells in Fanconi's anemia.
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pubmed:affiliation |
G. Prodi Interdepartmental Center for Cancer Research, University of Bologna, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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