Linked Life Data

8398820

Source:http://linkedlifedata.com/resource/pubmed/id/8398820

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1993-11-4
pubmed:abstractText
Investigations into the nature of the molecular interactions mediating the recognition of the haemopoietic progenitor cells by the haemopoietic stroma, indicate that ubiquitin mediates the binding between murine haemopoietic progenitors and the haemopoietic stroma. The adhesion of haemopoietic progenitors to anti-ubiquitin antibody treated bone marrow stroma cultures, shows inhibition of binding by approximately 78%. Affinity purification of the 1% Triton X-100 soluble stromal membrane fraction, on anti-ubiquitin-sepharose revealed a ubiquitinated 55 kD subunit. Progenitor cells treated with ubiquitin show approximately 58% inhibition in their ability to home into spleen, indicating the direct involvement of ubiquitin in homing. Histochemical staining of bone marrow cells using ubiquitin as a probe further delineates a population of cells possessing specific binding sites for ubiquitin. We demonstrate here the presence of a ubiquitin binding site on the haemopoietic progenitor cells, which may play a major role in the targeting of such progenitors to their 'niche' within the haemopoietic tissue. Such ubiquitin-mediated recognition may thus constitute a common molecular mechanism for homing and adhesion to both bone marrow and spleen, and may be implicated in the homing of more primitive, less differentiated haemopoietic progenitors. The results also indicate that the homing of haemopoietic progenitors within the haemopoietic micro-environment may be mediated by both a ubiquitin dependent and another ubiquitin independent mechanism.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0007-1048
pubmed:author
pubmed:issnType
Print
pubmed:volume
84
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
212-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Demonstration of a ubiquitin binding site on murine haemopoietic progenitor cells: implication of ubiquitin in homing and adhesion.
pubmed:affiliation
Span Research Centre, Surat, India.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't