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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
27
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pubmed:dateCreated |
1993-10-20
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pubmed:abstractText |
Receptors for peptide YY (PYY) were identified in the PKSV-PCT renal proximal tubule cell line, derived from transgenic mice (SV40 large T antigen under the control of the rat L-type pyruvate kinase 5'-regulatory sequence). Binding of [125I-Tyr36]monoiodo-PYY ([125I] PYY to cell was specific, saturable, and reversible. The order of potency for peptides for inhibiting [125I]PYY binding was: PYY > neuropeptide Y (NPY) = PYY (13-36) >> pancreatic polypeptide. A single class of receptors was observed with a Kd of 0.37 +/- 0.05 nM and a Bmax of 103 +/- 10 fmol/mg protein. After cross-linking, electrophoresis of covalent [125I]PYY-receptor complexes revealed a single band of M(r) 50,000. PYY receptors were exclusively present at the basolateral membrane surface of polarized cells and were coupled negatively to adenylylcyclase by a pertussis toxin-sensitive G protein. PKSV-PCT cell growth and T antigen expression could be modulated by D-glucose in the medium. PYY receptors were exclusively expressed in proliferative cells cultured in the presence of D-glucose. PYY receptors disappeared in the absence of D-glucose and were expressed again when proliferation was activated by reintroduction of D-glucose. PYY stimulated cell growth (17-26% increase) and promoted [methyl-3H]thymidine incorporation into DNA (64% increase; ED50 = 5 nM PYY) of cells grown in D-glucose-enriched medium. This latter effect of PYY was largely reversed by pretreatment of cells with pertussis toxin. These findings suggest that PYY receptors play a role in epithelial cell growth.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenylate Cyclase,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Polyomavirus Transforming,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Forskolin,
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Gastrointestinal Hormones,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide YY,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Pyruvate Kinase,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Gastrointestinal Hormone,
http://linkedlifedata.com/resource/pubmed/chemical/Thymidine,
http://linkedlifedata.com/resource/pubmed/chemical/peptide YY receptor
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
268
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
20547-54
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8397209-Adenylate Cyclase,
pubmed-meshheading:8397209-Animals,
pubmed-meshheading:8397209-Antigens, Polyomavirus Transforming,
pubmed-meshheading:8397209-Blotting, Western,
pubmed-meshheading:8397209-Cell Division,
pubmed-meshheading:8397209-Cell Line,
pubmed-meshheading:8397209-Cell Membrane,
pubmed-meshheading:8397209-Cyclic AMP,
pubmed-meshheading:8397209-DNA,
pubmed-meshheading:8397209-Forskolin,
pubmed-meshheading:8397209-GTP-Binding Proteins,
pubmed-meshheading:8397209-Gastrointestinal Hormones,
pubmed-meshheading:8397209-Gene Expression,
pubmed-meshheading:8397209-Glucose,
pubmed-meshheading:8397209-Kidney Tubules, Proximal,
pubmed-meshheading:8397209-Kinetics,
pubmed-meshheading:8397209-Mice,
pubmed-meshheading:8397209-Mice, Transgenic,
pubmed-meshheading:8397209-Molecular Weight,
pubmed-meshheading:8397209-Peptide YY,
pubmed-meshheading:8397209-Peptides,
pubmed-meshheading:8397209-Pyruvate Kinase,
pubmed-meshheading:8397209-Rats,
pubmed-meshheading:8397209-Receptors, Cell Surface,
pubmed-meshheading:8397209-Receptors, Gastrointestinal Hormone,
pubmed-meshheading:8397209-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:8397209-Simian virus 40,
pubmed-meshheading:8397209-Thymidine
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pubmed:year |
1993
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pubmed:articleTitle |
Peptide YY receptors in the proximal tubule PKSV-PCT cell line derived from transgenic mice. Relation with cell growth.
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pubmed:affiliation |
Unité de Biologie et Physiologie des Cellules Digestives, Institut National de la Santé et de la Recherche Médicale, Unité 239, Paris, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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