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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
27
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pubmed:dateCreated |
1993-10-20
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pubmed:databankReference |
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pubmed:abstractText |
Xenopus oocytes exhibit a receptor-evoked Cl- current that is mediated through the activation of phospholipase C (PLC) and release of intracellular Ca2+. The identity of PLC(s) mediating this effect is unknown. We have cloned cDNAs encoding a new form of PLC-beta from a Xenopus oocyte cDNA library. The Xenopus PLC-beta has substantial (33-64%) homology with mammalian beta 1, beta 2, beta 3, and beta 4 phospholipase C and is closest to PLC-beta 3, with 64% identity and 80% similarity. Injection of antisense oligonucleotides to a specific region of Xenopus PLC-beta results in degradation of its mRNA and significantly reduces Cl- currents evoked by both endogenous angiotensin receptors and expressed mammalian alpha 1b-adrenergic receptors and M1-muscarinic receptors as compared to responses in sense oligonucleotide-injected oocytes. Inhibition of the M1-muscarinic response by antisense oligonucleotides was nonadditive with pertussis toxin inhibition. PLC antisense oligonucleotide-injected oocytes show Cl- current responses to IP3 that are indistinguishable from sense oligonucleotide-injected oocytes. Since the receptor responses are pertussis toxin-sensitive, we conclude that we have isolated a new form of PLC-beta involved in the pertussis toxin-sensitive receptor stimulation of the Ca2+ activated Cl- current in Xenopus oocytes.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Chloride Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Chlorides,
http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Oligodeoxyribonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotides, Antisense,
http://linkedlifedata.com/resource/pubmed/chemical/Pertussis Toxin,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Adrenergic, alpha,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Angiotensin,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Muscarinic,
http://linkedlifedata.com/resource/pubmed/chemical/Type C Phospholipases,
http://linkedlifedata.com/resource/pubmed/chemical/Virulence Factors, Bordetella
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
268
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
19915-8
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:8397190-Amino Acid Sequence,
pubmed-meshheading:8397190-Animals,
pubmed-meshheading:8397190-Base Sequence,
pubmed-meshheading:8397190-Chloride Channels,
pubmed-meshheading:8397190-Chlorides,
pubmed-meshheading:8397190-Cloning, Molecular,
pubmed-meshheading:8397190-Drosophila,
pubmed-meshheading:8397190-Female,
pubmed-meshheading:8397190-Gene Library,
pubmed-meshheading:8397190-Isoenzymes,
pubmed-meshheading:8397190-Mammals,
pubmed-meshheading:8397190-Membrane Proteins,
pubmed-meshheading:8397190-Molecular Sequence Data,
pubmed-meshheading:8397190-Oligodeoxyribonucleotides,
pubmed-meshheading:8397190-Oligonucleotides, Antisense,
pubmed-meshheading:8397190-Oocytes,
pubmed-meshheading:8397190-Pertussis Toxin,
pubmed-meshheading:8397190-RNA, Messenger,
pubmed-meshheading:8397190-Receptors, Adrenergic, alpha,
pubmed-meshheading:8397190-Receptors, Angiotensin,
pubmed-meshheading:8397190-Receptors, Muscarinic,
pubmed-meshheading:8397190-Type C Phospholipases,
pubmed-meshheading:8397190-Virulence Factors, Bordetella,
pubmed-meshheading:8397190-Xenopus
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pubmed:year |
1993
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pubmed:articleTitle |
Receptor-evoked Cl- current in Xenopus oocytes is mediated through a beta-type phospholipase C. Cloning of a new form of the enzyme.
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pubmed:affiliation |
Department of Pharmacology, Mount Sinai School of Medicine, City University of New York, New York 10029.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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