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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
7
|
pubmed:dateCreated |
1993-10-21
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pubmed:abstractText |
We have cloned a cDNA that encodes the sodium pump alpha-subunit of the cat flea Ctenocephalides felis. The deduced amino acid sequence of 1037 residues is 90% homologous with the Drosophila alpha-subunit sequence and shows 75% similarity to the sheep kidney alpha-subunit. One characteristic unique to the insect polypeptides is an extended sequence at the N-terminus. Northern blot analysis of C. felis RNA detects two alpha-subunit transcripts with lengths of 3.8 and 4.4 kb. Sodium pump alpha-subunit sequences were readily detected in homogenates of flea midguts and in cDNA synthesized from midgut RNA.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0965-1748
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
23
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
809-14
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:8397036-Amino Acid Sequence,
pubmed-meshheading:8397036-Animals,
pubmed-meshheading:8397036-Base Sequence,
pubmed-meshheading:8397036-Cloning, Molecular,
pubmed-meshheading:8397036-DNA,
pubmed-meshheading:8397036-DNA Probes,
pubmed-meshheading:8397036-Digestive System,
pubmed-meshheading:8397036-Molecular Sequence Data,
pubmed-meshheading:8397036-Sequence Homology, Amino Acid,
pubmed-meshheading:8397036-Siphonaptera,
pubmed-meshheading:8397036-Sodium-Potassium-Exchanging ATPase
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pubmed:year |
1993
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pubmed:articleTitle |
Cloning and sequence analysis of the alpha subunit of the cat flea sodium pump.
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pubmed:affiliation |
Paravax Inc., Mountain View, Calif.
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pubmed:publicationType |
Journal Article,
Comparative Study
|