Linked Life Data

8396819

Source:http://linkedlifedata.com/resource/pubmed/id/8396819

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1993-10-14
pubmed:abstractText
The replication of Epstein-Barr virus (EBV) and the expression of EBV early proteins were studied in the Burkitt's lymphoma cell line Akata stimulated with anti-human immunoglobulin G antibody (anti-IgG). Akata cells contained approximately 20 copies of EBV genome per cell as covalently closed, circular DNA. EBV DNA replication was observed at 6 hr and reached a maximal level at 24 hr after treatment with anti-IgG. Virion DNA was found in the culture medium at 12 hr. The kinetics of expression of BMRF1 gene product (early antigen diffuse component; EA-D) paralleled that of EBV deoxyribonuclease (DNase) and of DNA polymerase. Immunoblotting analysis showed that three polypeptides with molecular masses of 54, 52, and 49 kilodaltons (kDa) were recognized as EA-D components. The EBV DNase polypeptide was detected by immunoblotting at 53 kDa. The anti-EBV DNA polymerase antibody recognized 120- and 54-kDa polypeptides in the Akata cells. Immunoprecipitation followed by immunoblotting showed that EA-D and EBV DNase polypeptides were coimmunoprecipitated with anti-EBV DNase antibody and with anti-EA-D monoclonal antibody. These findings indicate that EA-D forms a complex with EBV DNase polypeptide. The molecules of EA-D, EBV DNase, and DNA polymerase appear to be closely associated together on the EBV replication.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0042-6822
pubmed:author
pubmed:issnType
Print
pubmed:volume
196
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
900-4
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Epstein-Barr virus (EBV) replication and expressions of EA-D (BMRF1 gene product), virus-specific deoxyribonuclease, and DNA polymerase in EBV-activated Akata cells.
pubmed:affiliation
Department of Pharmacology, University of Massachusetts Medical School, Worcester 01655.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.