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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1993-10-14
|
| pubmed:databankReference | |
| pubmed:abstractText |
Four RNA transcripts from chicken embryo fibroblast cells infected with Marek's disease virus (MDV) strain 281Ml/1 hybridized to the 4-kbp MDV replicon DNA. In an attempt to identify open reading frames coding for the four transcripts, we determined the nucleotide sequences of 4-kbp replicon DNA (represents a single monomeric repeat unit of defective MDV genome). Computer analysis indicates that the 4-kbp MDV replicon DNA contains two intact open reading frames (ORFs) with common promoter regulatory elements. ORF-A codes for a putative 204 amino acid protein that shares 21 and 36% amino acid sequence identity to nuclear DNA binding proteins such as the EBNA-1 of Epstein-Barr virus and galline, a chicken sperm histone protein, respectively. ORF-B encodes for a potential 350 amino acid protein, which did not show any significant amino acid sequence identity to known protein sequences within Swiss-Protein data base. ORF-B may, therefore, encode a MDV specific protein. The 5'-region of MDV replicon DNA revealed seven reiterated copies of an 11-bp motif sharing 8 out of 11 nucleotide sequence identity to DR2 elements of the herpes simplex virus strain USA-8 a sequence.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0042-6822
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
196
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
484-95
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8396799-Amino Acid Sequence,
pubmed-meshheading:8396799-Animals,
pubmed-meshheading:8396799-Base Sequence,
pubmed-meshheading:8396799-Chickens,
pubmed-meshheading:8396799-Chromosome Mapping,
pubmed-meshheading:8396799-Cloning, Molecular,
pubmed-meshheading:8396799-DNA-Binding Proteins,
pubmed-meshheading:8396799-Defective Viruses,
pubmed-meshheading:8396799-Fibroblasts,
pubmed-meshheading:8396799-Genes, Viral,
pubmed-meshheading:8396799-Genome, Viral,
pubmed-meshheading:8396799-Herpesvirus 2, Gallid,
pubmed-meshheading:8396799-Molecular Sequence Data,
pubmed-meshheading:8396799-Open Reading Frames,
pubmed-meshheading:8396799-RNA, Messenger,
pubmed-meshheading:8396799-Replicon,
pubmed-meshheading:8396799-Sequence Analysis, DNA,
pubmed-meshheading:8396799-Sequence Homology, Amino Acid,
pubmed-meshheading:8396799-Simplexvirus,
pubmed-meshheading:8396799-Viral Proteins,
pubmed-meshheading:8396799-Virus Replication
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Defective Marek's disease virus DNA contains a gene encoding a potential nuclear DNA binding protein and a HSV a-like sequence.
|
| pubmed:affiliation |
Department of Animal Science, Michigan State University, East Lansing 48824.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|