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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1993-10-8
|
| pubmed:abstractText |
C.B-17 SCID mice were inoculated with human peripheral blood leukocytes (PBLs) from normal Epstein-Barr virus (EBV)-seropositive and -seronegative donors. Confirmation of a functioning human immune response was demonstrated by the detection of human antibody after inoculation with rotavirus, tetanus toxoid, or EBV. One group of animals inoculated with PBLs from an EBV-seropositive donor developed immunoblastic lymphomas approximately 9 weeks after transplantation. Confirmation of the species and sex of origin of the tumor cells was established using a spontaneous cell line prepared from the tumor. At passage I, the tumor-cell line (AGTI) showed 15% of the metaphases with a translocation involving chromosomes 5 and 14. A lymphoblastoid cell line (AGLCL) established from the same PBLs from the same donor at the time of inoculation of the mice had a normal female karyotype. The AGLCL and a clone of AGTI cells were analyzed for rearrangement of immunoglobulin heavy chain (IgH) genes; both cell lines showed rearrangement of both IgH alleles. The results outlined in this report suggest that a spontaneous chromosomal translocation involving chromosome 14 occurred in normal PBLs in the SCID mouse.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Epstein-Barr Virus Nuclear Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0020-7136
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
9
|
| pubmed:volume |
55
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
281-7
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:8396561-Animals,
pubmed-meshheading:8396561-Antibodies, Viral,
pubmed-meshheading:8396561-Antigens, Viral,
pubmed-meshheading:8396561-Chromosomes, Human, Pair 14,
pubmed-meshheading:8396561-Chromosomes, Human, Pair 5,
pubmed-meshheading:8396561-DNA-Binding Proteins,
pubmed-meshheading:8396561-Epstein-Barr Virus Nuclear Antigens,
pubmed-meshheading:8396561-Female,
pubmed-meshheading:8396561-Gene Rearrangement, B-Lymphocyte, Heavy Chain,
pubmed-meshheading:8396561-Genes, Immunoglobulin,
pubmed-meshheading:8396561-Herpesvirus 4, Human,
pubmed-meshheading:8396561-Humans,
pubmed-meshheading:8396561-Immunoglobulin G,
pubmed-meshheading:8396561-Lymphoma, B-Cell,
pubmed-meshheading:8396561-Mice,
pubmed-meshheading:8396561-Mice, SCID,
pubmed-meshheading:8396561-Neoplasm Transplantation,
pubmed-meshheading:8396561-Translocation, Genetic,
pubmed-meshheading:8396561-Tumor Cells, Cultured,
pubmed-meshheading:8396561-Tumor Virus Infections
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Spontaneous development of a chromosomal translocation 5;14 in an Epstein-Barr-virus-associated B-cell lymphoma in a SCID mouse.
|
| pubmed:affiliation |
Department of Medical Microbiology and Immunology, Ohio State University College of Medicine, Columbus 43210.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|