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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
1993-10-5
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pubmed:abstractText |
During the period May 1987-January 1989, faecal samples from 417 paediatric inpatients admitted to the main paediatric hospital in Rome were screened by direct electron microscopy and rotavirus enzyme-linked immunosorbent assay. Rotaviruses were detected in 18.2% of cases and adenoviruses in 7%, whereas astroviruses were found in 1% of cases. Different percentages of rotavirus excretors were revealed by enzyme-linked immunosorbent assay and electron microscopy. This discrepancy seems to be due to false positive results introduced by enzyme-linked immunosorbent assay. Analysis of electron microscopy-positive samples by rotaviral RNA polyacrylamide gel electrophoresis showed different electropherotypes of rotavirus among which a single, largely predominant long electropherotype (55.4%) was revealed. Short electropherotype subgroup I rotaviruses were demonstrated in about 10.7% of samples.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1121-7138
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
16
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
215-25
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pubmed:dateRevised |
2011-2-25
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pubmed:meshHeading |
pubmed-meshheading:8396194-Adenovirus Infections, Human,
pubmed-meshheading:8396194-Child, Preschool,
pubmed-meshheading:8396194-Diarrhea,
pubmed-meshheading:8396194-Diarrhea, Infantile,
pubmed-meshheading:8396194-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:8396194-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:8396194-Humans,
pubmed-meshheading:8396194-Infant,
pubmed-meshheading:8396194-Infant, Newborn,
pubmed-meshheading:8396194-Mamastrovirus,
pubmed-meshheading:8396194-Microscopy, Electron,
pubmed-meshheading:8396194-Picornaviridae Infections,
pubmed-meshheading:8396194-RNA, Viral,
pubmed-meshheading:8396194-Rome,
pubmed-meshheading:8396194-Rotavirus Infections,
pubmed-meshheading:8396194-Sensitivity and Specificity,
pubmed-meshheading:8396194-Virus Diseases
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pubmed:year |
1993
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pubmed:articleTitle |
Viral childhood diarrhoea in Rome: a diagnostic and epidemiological study.
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pubmed:affiliation |
Laboratorio di Ultrastrutture, Istituto Superiore di Sanità, Rome, Italy.
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pubmed:publicationType |
Journal Article
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