Linked Life Data

8393961

Source:http://linkedlifedata.com/resource/pubmed/id/8393961

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1993-9-3
pubmed:abstractText
Experiments were designed to examine the effects of trypsin-mediated proteolysis on the activity of the rabbit renal brush border membrane Na(+)-H+ exchanger. Incubation of brush border membrane vesicles with trypsin resulted in a concentration and time-dependent change in proton gradient-stimulated sodium uptake. Extensive trypsin digestion resulted in a marked decrease in the activity of the transporter. By contrast, limited trypsin digestion resulted in a significant increase in proton gradient-stimulated, amiloride-inhibitable sodium uptake. Sodium-dependent proton efflux was also significantly increased by limited trypsin digestion. The passive permeability of the vesicles to sodium and protons, and the sodium-dependent and sodium-independent uptake of glucose were not affected by limited trypsin digestion. These data indicate that limited trypsin digestion increases the activity of the renal brush border membrane Na(+)-H+ exchanger. It is suggested that the renal Na(+)-H+ exchanger contains one or more inhibitory components or sites which are sensitive to inactivation by limited trypsin-mediated proteolysis.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0378-0392
pubmed:author
pubmed:issnType
Print
pubmed:volume
19
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
47-50
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Trypsin digestion increases Na(+)-H+ exchange rates in native rabbit brush border membrane.
pubmed:affiliation
Department of Medicine, University of California, Los Angeles.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't