rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
2
|
pubmed:dateCreated |
1993-8-26
|
pubmed:abstractText |
Cultured astrocytes from the cerebral cortex of the rat respond to opioid kappa-receptor stimulation with a substantial elevation of the cytoplasmic free calcium, visualized through the use of the fluorescent calcium indicator Fura-2. The stimulation of kappa-receptors with U-50488H increases the level of calcium through a dose-related stimulatory effect on the transmembrane calcium influx. The kappa-receptor stimulation was completely blocked by the selective kappa-receptor blocker nor-binaltorphimine. Furthermore, the transmembrane calcium influx was completely blocked by nifedipine, indicating the involvement of L-type channels. The presence of L-type channels was verified by stimulation of L-type channels with Bay K8644. The effects of Bay K8644 were completely blocked by nifedipine. L-type channel-coupled kappa-receptors on astrocytes might represent a novel mechanism contributing to the depressant action of opioids on synaptic transmission via decreasing the availability of extracellular calcium necessary for presynaptic transmitter release.
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pubmed:language |
eng
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pubmed:journal |
|
pubmed:citationSubset |
IM
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pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
May
|
pubmed:issn |
0306-4522
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
54
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
401-7
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:8393154-3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benz...,
pubmed-meshheading:8393154-Analgesics,
pubmed-meshheading:8393154-Animals,
pubmed-meshheading:8393154-Animals, Newborn,
pubmed-meshheading:8393154-Astrocytes,
pubmed-meshheading:8393154-Calcium,
pubmed-meshheading:8393154-Calcium Channels,
pubmed-meshheading:8393154-Cells, Cultured,
pubmed-meshheading:8393154-Cerebral Cortex,
pubmed-meshheading:8393154-Glial Fibrillary Acidic Protein,
pubmed-meshheading:8393154-Immunohistochemistry,
pubmed-meshheading:8393154-Kinetics,
pubmed-meshheading:8393154-Pyrrolidines,
pubmed-meshheading:8393154-Rats,
pubmed-meshheading:8393154-Rats, Sprague-Dawley,
pubmed-meshheading:8393154-Receptors, Opioid, kappa,
pubmed-meshheading:8393154-Time Factors
|
pubmed:year |
1993
|
pubmed:articleTitle |
Kappa-opioid receptors on astrocytes stimulate L-type Ca2+ channels.
|
pubmed:affiliation |
Institute of Neurobiology, University of Göteborg, Sweden.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|