Linked Life Data

8393147

Source:http://linkedlifedata.com/resource/pubmed/id/8393147

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6437
pubmed:dateCreated
1993-8-26
pubmed:abstractText
Low-molecular-weight GTP-binding proteins (small G proteins) of the Rab family have been proposed to act as central regulators of vesicular traffic, and proteins of the Rab3 subfamily (Rab3A, B, C and D) are thought to be associated with membrane vesicles or granules undergoing exocytotic fusion with the plasma membrane. Rab3A is highly expressed in brain, whereas Rab3B is the major form found in rat anterior pituitary gland. We report here that antisense oligonucleotides against Rab3B, introduced into pituitary cells using the whole-cell patch clamp technique, specifically and reversibly block expression of Rab3B. We find that calcium-dependent exocytosis is inhibited without affecting endocytosis. Antisense oligonucleotides directed against Rab3A have no effect. Our results indicate that Rab3B is likely to be a key intracellular signalling molecule which can control exocytosis downstream of other calcium-dependent processes in anterior pituitary cells.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0028-0836
pubmed:author
pubmed:issnType
Print
pubmed:day
5
pubmed:volume
364
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
540-4
pubmed:dateRevised
2009-9-29
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Inhibition of Rab3B expression attenuates Ca(2+)-dependent exocytosis in rat anterior pituitary cells.
pubmed:affiliation
Department of Neurobiology, AFRC Babraham Institute, Cambridge, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't