Linked Life Data

8392709

Source:http://linkedlifedata.com/resource/pubmed/id/8392709

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1993-8-17
pubmed:abstractText
mRNA analogues containing 4-thiouridine residues at selected sites were used to extend our analysis of photo-induced cross-links between mRNA and 16S RNA to cover the entire downstream range between positions +1 and +16 on the mRNA (position +1 is the 5'-base of the P-site codon). No tRNA-dependent cross-links were observed from positions +1, +2, +3 or +5. Position +4 on the mRNA was cross-linked in a tRNA-dependent manner to 16S RNA at a site between nucleotides ca 1402-1415 (most probably to the modified residue 1402), and this was absolutely specific for the +4 position. Similarly, the previously observed cross-link to nucleotide 1052 was absolutely specific for the +6 position. The previously observed cross-links from +7 to nucleotide 1395 and from +11 to 532 were however seen to a lesser extent with certain types of mRNA sequence from neighbouring positions (+6 to +10, and +10 to +13, respectively); no tRNA-dependent cross-links to other sites on 16S RNA were found from these positions, and no cross-linking was seen from positions +14 to +16. In each case the effect of a second cognate tRNA (at the ribosomal A-site) on the level of cross-linking was studied, and the specificity of each cross-link was confirmed by translocation experiments with elongation factor G, using appropriate mRNA analogues.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8392709-1379176, http://linkedlifedata.com/resource/pubmed/commentcorrection/8392709-1531520, http://linkedlifedata.com/resource/pubmed/commentcorrection/8392709-1610836, http://linkedlifedata.com/resource/pubmed/commentcorrection/8392709-1651232, http://linkedlifedata.com/resource/pubmed/commentcorrection/8392709-1712292, http://linkedlifedata.com/resource/pubmed/commentcorrection/8392709-2251116, http://linkedlifedata.com/resource/pubmed/commentcorrection/8392709-2443708, http://linkedlifedata.com/resource/pubmed/commentcorrection/8392709-2451022, http://linkedlifedata.com/resource/pubmed/commentcorrection/8392709-2464693, http://linkedlifedata.com/resource/pubmed/commentcorrection/8392709-2664777, http://linkedlifedata.com/resource/pubmed/commentcorrection/8392709-2690012, http://linkedlifedata.com/resource/pubmed/commentcorrection/8392709-8422963
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0305-1048
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2853-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Site-directed cross-linking of mRNA analogues to 16S ribosomal RNA; a complete scan of cross-links from all positions between '+1' and '+16' on the mRNA, downstream from the decoding site.
pubmed:affiliation
Max-Planck-Institut für Molekulare Genetik, Abteilung Wittmann, Berlin, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't