Linked Life Data

8392648

Source:http://linkedlifedata.com/resource/pubmed/id/8392648

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1993-8-16
pubmed:abstractText
Hypoxia remains an important clinical problem and affects neutrophil oxygen-dependent microbicidal pathways. For adequate PMN-cidal activity to occur, two sets of opsonic receptors (FcR, CD16, CD32w; complement receptors, CD35, CD11b/CD18) must be expressed on the cell surface. We hypothesized that hypoxia would adversely affect receptor expression and that the biological surface that the PMN were adhered to would modulate the effect of hypoxia on these receptors. PMN were adhered in the presence of buffer, fibronectin, Arg-Gly-Asp-Ser (RGDS), or laminin followed by assessment of PMN FcR and complement receptors using 125I-labeled monoclonal antibodies directed against these receptors. Hypoxia reduced PMN CD16 and CD32w but not CD35 and CD11b/CD18 expression. Decreasing buffer pO2 led to corresponding decreases in CD16 and CD32w expression. RGDS but not fibronectin or laminin restored CD16 and CD32w expression in the presence of hypoxia. Monensin but not cycloheximide inhibited RGDS restoration of CD16 and CD32w. (cpm bound: CD16, 958 +/- 123 vs 1602 +/- 193; CD32w, 1481 +/- 173 vs 2215 +/- 382 for hypoxia buffer+RGDS +/- monensin.) These results demonstrate that: (1) acute hypoxia creates an opsonic mismatch by reducing CD16 and CD32w without affecting complement receptors CR1 and CR3 (CD35, CD11b/CD18); (2) matrix proteins modulate the effect of acute hypoxia on PMN FcR; (3) the RGDS-binding epitope of fibronectin significantly restores PMN FcR in the face of acute hypoxia; and (4) RGDS upregulates FcR expression during acute hypoxia by increasing receptor recycling to the cell surface.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0022-4804
pubmed:author
pubmed:issnType
Print
pubmed:volume
54
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
299-304
pubmed:dateRevised
2005-11-17
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Hypoxia induces an opsonic mismatch on the polymorphonuclear leukocyte surface-reversal via Arg-Gly-Asp-Ser-mediated adhesion.
pubmed:affiliation
Department of Surgery, Brown University School of Medicine/Rhode Island Hospital, Providence 02903.
pubmed:publicationType
Journal Article