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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1993-8-6
|
| pubmed:abstractText |
We developed a quantitative method of hepatitis C virus RNA by competitive reverse transcription-polymerase chain reaction. With this method, 36 patients with type C chronic liver disease were analyzed for the copy number of circulating hepatitis C virus in 50 microliters of serum. The amounts of hepatitis C virus RNA ranged from 10(1) to 10(7) copies in the 36 patients. The average amount of hepatitis C virus RNA was 10(3.3 +/- 2.2) copies in 12 patients with chronic persistent hepatitis, 10(5.7 +/- 1.6) copies in 12 patients with chronic active hepatitis and 10(6.0 +/- 1.6) copies in 12 patients with cirrhosis (including 4 patients with hepatocellular carcinoma). The amount of hepatitis C virus RNA in serum was significantly less in patients with chronic persistent hepatitis than in patients with chronic active hepatitis or cirrhosis (p < 0.01), and it tended to increase according to the progression of histopathological changes of the liver. Furthermore, it was revealed that the amount of hepatitis C virus RNA became exponentially larger as the term from infection became longer. Quantification of hepatitis C virus RNA by competitive reverse transcription-polymerase chain reaction may have many applications for the study of clinical features of hepatitis C virus infection.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0270-9139
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
18
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
16-20
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:8392026-Adult,
pubmed-meshheading:8392026-Aged,
pubmed-meshheading:8392026-Base Sequence,
pubmed-meshheading:8392026-Carcinoma, Hepatocellular,
pubmed-meshheading:8392026-Chronic Disease,
pubmed-meshheading:8392026-Deoxyribonuclease EcoRI,
pubmed-meshheading:8392026-Female,
pubmed-meshheading:8392026-Hepacivirus,
pubmed-meshheading:8392026-Hepatitis, Chronic,
pubmed-meshheading:8392026-Hepatitis C,
pubmed-meshheading:8392026-Humans,
pubmed-meshheading:8392026-Liver Cirrhosis,
pubmed-meshheading:8392026-Liver Neoplasms,
pubmed-meshheading:8392026-Male,
pubmed-meshheading:8392026-Middle Aged,
pubmed-meshheading:8392026-Molecular Sequence Data,
pubmed-meshheading:8392026-Polymerase Chain Reaction,
pubmed-meshheading:8392026-RNA, Viral
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Quantification of hepatitis C virus by competitive reverse transcription-polymerase chain reaction: increase of the virus in advanced liver disease.
|
| pubmed:affiliation |
First Department of Medicine, Chiba University School of Medicine, Japan.
|
| pubmed:publicationType |
Journal Article
|