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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1993-8-12
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pubmed:databankReference |
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/12685,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L03653,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L03654,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L05530,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L07632,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L12685,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L12686,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M96931,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M96932,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M96933,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X54209
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pubmed:abstractText |
Five protein families are needed to encompass the diversity of cyclic-AMP (cAMP) phosphodiesterases (PDE). Family IV PDEs (PDE IV) specifically hydrolyze cAMP with a low Km, and are selectively inhibited by rolipram (Rp) and related drugs. Cloned cDNAs from rat (r) suggest that the PDE IV family comprises four distinct members, designated A, B, C and D. Using RN from a human lymphocytic B-cell line (43D-Cl2), we have isolated a 3.8-kb cDNA by low-stringency screening using a rat PDE IV member B (r-PDE IVB) probe. Expression of the human (h) cDNA in Escherichia coli results in cAMP-specific PDE activity that is Rp sensitive. A single large open reading frame (ORF) predicts a 564-amino-acid protein with 92.9% identity to r-PDE IVB; at the nucleotide level the identity is 86.3%. This h-PDE IVB clone, HPB106, differs from a related cDNA clone isolated by others from h-monocytes [Livi et al., Mol. Cell. Biol. 10 (1990) 2678-2686]. Our analysis identifies the monocyte clone with r-PDE IVA. Southern blots using a 1.2-kb h-PDE IVB probe at low stringency suggest the presence of additional uncloned human PDE IV family members. Analysis of genomic Southern blots using short specific probes from the h-PDE IVA and h-PDE IVB cDNAs indicates that distinct genes encode these two PDE IV family members. RNA from fractionated normal human leukocytes shows major specific messages of 3.0 and 4.6 kb for h-PDE IVA and 3.7 kb for h-PDE IVB.(ABSTRACT TRUNCATED AT 250 WORDS)
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0378-1119
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
30
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pubmed:volume |
129
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
239-47
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:8392015-3',5'-Cyclic-AMP Phosphodiesterases,
pubmed-meshheading:8392015-Alternative Splicing,
pubmed-meshheading:8392015-Amino Acid Sequence,
pubmed-meshheading:8392015-Animals,
pubmed-meshheading:8392015-B-Lymphocytes,
pubmed-meshheading:8392015-Base Sequence,
pubmed-meshheading:8392015-Binding Sites,
pubmed-meshheading:8392015-Blotting, Northern,
pubmed-meshheading:8392015-Blotting, Southern,
pubmed-meshheading:8392015-Cell Line,
pubmed-meshheading:8392015-DNA, Recombinant,
pubmed-meshheading:8392015-Escherichia coli,
pubmed-meshheading:8392015-Genetic Variation,
pubmed-meshheading:8392015-Humans,
pubmed-meshheading:8392015-Isoenzymes,
pubmed-meshheading:8392015-Molecular Sequence Data,
pubmed-meshheading:8392015-Multigene Family,
pubmed-meshheading:8392015-Polymerase Chain Reaction,
pubmed-meshheading:8392015-Protein Processing, Post-Translational,
pubmed-meshheading:8392015-Pyrrolidinones,
pubmed-meshheading:8392015-RNA, Messenger,
pubmed-meshheading:8392015-Rats,
pubmed-meshheading:8392015-Recombinant Fusion Proteins,
pubmed-meshheading:8392015-Rolipram,
pubmed-meshheading:8392015-Sequence Analysis, DNA,
pubmed-meshheading:8392015-Sequence Homology, Amino Acid
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pubmed:year |
1993
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pubmed:articleTitle |
The cDNA of a human lymphocyte cyclic-AMP phosphodiesterase (PDE IV) reveals a multigene family.
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pubmed:affiliation |
Syntex Discovery Research, Institute of Bio-Organic Chemistry, Palo Alto, CA 94304.
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pubmed:publicationType |
Journal Article
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