Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1993-8-11
|
| pubmed:abstractText |
We investigated 81 cases of peripheral pleomorphic T-cell lymphoma (PMTCL) occurring in human immunodeficiency virus-negative Europeans for the presence of Epstein-Barr virus (EBV)-DNA through polymerase chain reaction (PCR) for the presence of EBV-encoded small nuclear RNAs (EBER) and immediate early mRNAs (Bam H-fragment, lower strand frame [BHLF]) by in situ hybridization (ISH) and for EBV-encoded latent membrane protein (LMP) and nuclear antigen 2 (EBNA2) by immunohistology (IH). EBER-ISH, which could be applied on all cases, showed an overall incidence of EBV-infected cells in 38 of 81 cases (47%) of PMTCL. These data could be confirmed by PCR, which produced congruent results in the cases with amplifiable DNA. By EBER-ISH, the virus was located in the tumor cells in 30 of the 38 EBV-positive cases, with the proportion of the infected cells ranging from 1% to 100%. In 18 of these cases and in the 8 cases without EBV-infected tumor cells, the virus was, respectively, either additionally or exclusively detectable in occasional nonmalignant lymphoid bystander cells. An LMP expression was observed in several of the EBER-expressing tumor cells in 18 cases, whereas EBNA2 was detectable only in one case, which also displayed signs of viral replication. Some nonmalignant EBV-infected B immunoblasts also expressed LMP in several cases. Primary cutaneous and enteropathy-associated PMTCL displayed less frequent EBV infection when compared with other extranodal or nodal manifestations.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/EBV-associated membrane antigen...,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Matrix Proteins
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0006-4971
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
82
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
217-23
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8391874-Adult,
pubmed-meshheading:8391874-Aged,
pubmed-meshheading:8391874-Antigens, Viral,
pubmed-meshheading:8391874-B-Lymphocytes,
pubmed-meshheading:8391874-DNA, Viral,
pubmed-meshheading:8391874-Europe,
pubmed-meshheading:8391874-Female,
pubmed-meshheading:8391874-Herpesvirus 4, Human,
pubmed-meshheading:8391874-Humans,
pubmed-meshheading:8391874-In Situ Hybridization,
pubmed-meshheading:8391874-Lymphoma, T-Cell, Peripheral,
pubmed-meshheading:8391874-Male,
pubmed-meshheading:8391874-Middle Aged,
pubmed-meshheading:8391874-Polymerase Chain Reaction,
pubmed-meshheading:8391874-RNA, Messenger,
pubmed-meshheading:8391874-RNA, Viral,
pubmed-meshheading:8391874-T-Lymphocytes,
pubmed-meshheading:8391874-Tumor Virus Infections,
pubmed-meshheading:8391874-Viral Matrix Proteins
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Frequent latent Epstein-Barr virus infection of neoplastic T cells and bystander B cells in human immunodeficiency virus-negative European peripheral pleomorphic T-cell lymphomas.
|
| pubmed:affiliation |
Konsultations und Referenzzentrum für Lymphknotenund Hämatopathologie am Institut für Pathologie, Klinikum Steglitz, Freie Universität, Berlin, Germany.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|